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EC number: 421-320-0 | CAS number: 118289-55-7 CP-89,575
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 - 30 October 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Done under GLP and OECD methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Method C3, Directive 92/69/EEC
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- A study was conducted to assess the inhibitory effect of CP-89,575 on the growth of the unicellular green alga Selenastrum capricornutum, Strain No. CCAP 278/4.Triplicate algal cultures were exposed to ten saturated solutions of CP-89,575 in algal media, nominally ranging from 0.1, 0.22, 0.46, 1.0, 2.2, 4.6, 10, 22, 46 and 100%, plus one untreated control. Solutions were prepared by filtration and dilution of a saturated solution stirred overnight. The highest test level (100% saturated) was the undiluted filtrate. Cultures were incubated in a Gallenkamp Orbital Incubator under continuous illumination at 24°C for 72 hours. Cell numbers were counted daily to monitor growth
- GLP compliance:
- yes
Test material
- Reference substance name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- EC Number:
- 421-320-0
- EC Name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- Cas Number:
- 118289-55-7
- Molecular formula:
- Hill formula: C10 H9 Cl2 N O CAS formula: C10 H9 Cl2 N O
- IUPAC Name:
- 6-chloro-5-(2-chloroethyl)-2,3-dihydro-1H-indol-2-one
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Intended use: Pharmaceutical intermediate
Appearance: Off-white powder
Storage conditions: Room temperature in the dark
Expiry: 2 June 1999
Purity: 98.82% by hplc
Date received: 2 June 1997
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:Test concentrations were verified by chemical analysis. Duplicate medium samples (100 ml) were taken from the control and each test concentration at 0 and 72 hours for analysis
- Sampling method:Additional samples were also taken from flasks containing the test substance at a concentration equivalent to the highest exposure concentration but with no algae present, at 0 hours and at 72 hours (replicates pooled), in order to obtain information on the extent of adsorption/absorption of the test substance by the algal cells
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: acetonitrile : water (50:50 v/v)
Test organisms
- Test organisms (species):
- Scenedesmus capricornutum
- Details on test organisms:
- TEST ORGANISM
- Common name:Scenedesmus capricornutum
- Strain:CCAP 278/4
- Source (laboratory, culture collection):Culture Collection of Algae & Protozoa, Institute of Freshwater Ecology, Cumbria, UK.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Cell numbers were counted daily to monitor growth.
Test conditions
- Hardness:
- NO data
- Test temperature:
- The temperature was maintained at 24 ± 1°C
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Geometric mean measured concentrations: 0.0019, 0.0043, 0.0095, 0.022, 0.044, 0.077, 0.24,
0.61, 1.4and3.1 mg/1
Measured concentrations ranged from 86.1 to 103% of target dilution factor at 0 hours and 33.4 to 105% of target dilution factor at 72 hours. - Details on test conditions:
- Conical flasks (250 ml) each containing 100 ml of test or control culture were loosely stoppered and placed without conscious bias in a Gallenkamp Illuminated Orbital Incubator. The cultures were incubated, without medium renewal, for 72 hours under continuous illumination of approximately 7000 lux provided by 7 x 30 W "universal white" 1 metre fluorescent tubes. The temperature was maintained at 24 ± 1°C. Gaseous exchange and suspension of the algal cells were ensured by the action of the orbital shaker, oscillating at 120 cycles per minute
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.2 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95%
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 3.1 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.077 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Comparison of the measured concentrations of the test substance obtained from medium samples at a concentration equivalent to the highest test concentration, but without the addition of algal cells, indicated that the presence of algal cells had affected the stability of the exposure solutions.The
A
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 0-72hr ErC50 of test substance was calculated to be >3.1 mg/l and NOEC 0.077mg/l.
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