Methanesulfonic acid, 1,1'-(1,6-hexanediyldiimino)bis[1-oxo-, sodium salt (1:2)

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

An acute inhalation toxicity test was conducted with rats to determine the potential for the aerosolized dust of 1,1' -(1 ,6-Hexanediyldiimino)bis[l-oxo-methanesulfonic acid], sodium salt (1 :2) to produce toxicity via the inhalation (nose-only exposure) route. Ten healthy rats (5/sex) were exposed to the test atmosphere og 5.57 mh/m³ for 4 hours. The animals were observed for mortality, signs of gross toxicity, and behavioral changes at least once daily for 14 days following exposure. Body weights were recorded prior to exposure and again on days 1, 3, 7 and 14 (termination). Necropsies were performed on all animals at terminal sacrifice.

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Methansulfonic acid (1,6-hexanediyl-diimino)bis[1-oxo, disodium salt

EC Number:

690-526-2

Cas Number:

38632-47-2

Molecular formula:

C8H14S2N2O8.Na2

IUPAC Name:

Methansulfonic acid (1,6-hexanediyl-diimino)bis[1-oxo, disodium salt

Details on test material:

Test substance, identified as 1, 1' -(1 ,6-Hexanediyldiimino)bis[l-oxo-methanesulfonie acid], sodium salt (1:2)
Composition: 91.4%, Citric acid - 6.6%, Tween 20 - 2%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Gravimetric chamber concentration: 5.57 mg/L

No. of animals per sex per dose:

5 male and 5 female rats/dose

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.57 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Any other information on results incl. tables

All animals survived exposure to the test atmosphere. Following exposure all rats exhibited facial staining, nasal discharge and/or abnormal respiration. All animals recovered from the above symptoms by Day 2 and, apart from ocular discharge observed in one male on Days 8 and 9, appeared active and healthy for the remainder of the observation period. Although three males and two females lost or failed to gain body weight by Day 1, they showed a continued weight gain thereafter and along with all other animals gained weight through Day 14. No gross abnormalities were noted far any of the animals when necropsied at the conclusion of the 14-day observation period.

Applicant's summary and conclusion

Executive summary:

An acute inhalation toxicity test was conducted with rats to determine the potential for the aerosolized dust of 1,1' -(1 ,6-Hexane- diyldiimino)bis[l-oxo-methanesulfonic acid], sodium salt (1 :2) to produce toxicity via the inhalation (nose-only exposure) route. Under the conditions of this study, the single exposure acute inhalation LC50 ofthe test substance is greater than 5.57 mg/L in male and fe male rats.