Registration Dossier
Registration Dossier
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EC number: 203-565-3 | CAS number: 108-26-9
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline Study, non GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- adopted May 12th, 1981
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,4-dihydro-5-methyl-3H-pyrazol-3-one
- EC Number:
- 203-565-3
- EC Name:
- 2,4-dihydro-5-methyl-3H-pyrazol-3-one
- Cas Number:
- 108-26-9
- Molecular formula:
- C4H6N2O
- IUPAC Name:
- 2,4-dihydro-5-methyl-3H-pyrazol-3-one
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gaukler, Offenbach/Main, Germany
- Weight at study initiation: mean: males 3.27 kg, female 2.86 kg
- Housing: single
- Diet: ca. 130 g per animal
- Water: ca. 250 mL per animal
- Acclimation period: approx. 8 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): 30 - 70
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- other: untreated skin of the same animal
- Amount / concentration applied:
- 0.5 g of an 50% aqueous preparation
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 8 days
reading time points: 30 - 60 minutes after removing of test substance, 24, 48, 72 hours and 8 days after application - Number of animals:
- 3
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 x 2.5 cm, upper third of the back or flank
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes with Lutrol and Lutrol/water (1:1)
- Time after start of exposure: 4 hours
SCORING SYSTEM: according to guideline
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24, 48, 72 hours
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
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