Cyclopropanecarbonitrile, 1-amino-, hydrochloride (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Toxicity to aquatic algae was determined according to EU Method C.3 (Algae InhibitionTest)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

EC Number:

629-718-8

Cas Number:

127946-77-4

Molecular formula:

C4H7N2Cl

IUPAC Name:

1-Amino-1-cyclopropanecarbonitrile hydrochloride

Constituent 2

Reference substance name:

1-Cyanocycloproanaminium chloride

IUPAC Name:

1-Cyanocycloproanaminium chloride

Constituent 3

Reference substance name:

1-Aminocyclopropane-1-carbonitrile hydrochloride

IUPAC Name:

1-Aminocyclopropane-1-carbonitrile hydrochloride

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Initial Concentration of the test item : 99.8%
Sample storage conditions before analysis: in a closed vessel dark and dry at room temperature.
A stock solution containing 125.5mg/L test item in desionised water was prepared.

Test solutions

Vehicle:

yes

Details on test solutions:

From the stock solution (125.5mg/L of test item), the concentrations to be tested were prepared by diluting with desionised water.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Detail on test organism:
-Strain: CHODAT
-Family: Chlorophyceae
-Order: Chlorococcales
-Origin and culture: the culture was obtained in Oct.2011 by MBM Science-bridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 8°C.
-Acclimation period: four days before the start , an aliquot of the stock culture containing a few cells was brought into pre-culture medium and incubated for 96 hours. Before usage, the culture was checked on the absence of cell aggregates. After the adjustement to a cell concentration of about 3*10^4/mL through photometric measurement and addition of algal medium, the culture was usable for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

-

Test temperature:

26°C

pH:

The pH values in the control ranged from 7.8 to 7.8.
Detail in table 9.1-b of the study report.

Dissolved oxygen:

-

Salinity:

-

Nominal and measured concentrations:

Nominal concentrations: 0.32 / 1.0 / 3.2 / 10 / 32 / 100 mg/L.
For treatments 10-100mg/L the recovery after 72 hours was in range of 94-105% of the start concentration. Details in paragraph 9.1.4 of the study report.

Details on test conditions:

TEST SYSTEM
-Test vessel: glass flasks 50mL
-Material, size, headspace, fill volume: the test vessels were filld with 45+-5mL of the mixture and were incubated open for 72 hours shaken on an orbital shaker.
-Control end cells density: before the start (initial cells density: 4526 cell nber/mL) and every 24 hours the cells numbers were determined by photometric measurment of optical density. Values in table 9.1-a of the study report.
-Replicates: six for the controls and three for each treatment.

GROWTH MEDIUM
Standard medium used

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

growth rate

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

73 mg/L

Nominal / measured:

estimated

Basis for effect:

biomass

Details on results:

Microscopial observation:
From 0 to 32 mg/L: normal growth.
At 100 mg/L: no cells.

Results with reference substance (positive control):

-Results with reference substance valid? YES
-EC50: the EC50s of potassium dichromate were tested in a current reference test. The values lay within the normal range of the laboratory.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Results for 1-Aminocyclopropane-1-carbonitrile hydrochloride :
ECr50 >100mg/L
This result will not conduct to a classification "dangerous for the environment".