Registration Dossier
Registration Dossier
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EC number: 200-076-7 | CAS number: 51-03-6
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The effects on soil microflora were tested by Staebler, D. (2009) in a 28 days test. The test was conducted in a dose-response design with five test concentrations. The test was performed according to OECD 216 (2006) and OECD 217 (2000) guidelines. The EC50 of Piperonyl butoxide for nitrate formation rate (0 to 28 days) was calculated to be 332 mg/kg soil dry weight with a lower limit of 289 mg/kg soil dry weight and an upper limit of 381 mg/kg soil dry weight (95% confidence limits, Probit analysis) . The EC50 for short-term respiration was determined to be above 1000 mg/kg soil dry weight.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 332 mg/kg soil dw
Additional information
The study of Staebler, D. (2009) was conducted according to OECD 216 (2000) and OECD 217 (2000) guidelines and was found to be valid. The study is considered as "reliable without restriction".
The results are summarised in the following table:
| Endpoint | Value | Reference | |
EC50 (N-turnover 0 to 28 days) |
332 mg/kg soil dry weight |
Staebler, D. (2009) | |
EC50 (Short-term respiration)
|
>1000 mg/kg soil dry weight |
Staebler, D. (2009) | |
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