Aluminium metaphosphate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29.09.2014 - 16.12.2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein- Westfalen

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 6.25, 12.5, 25, 50, and 100 mg/L
- Sampling method: Samples were taken directly after test solution preparation and after 48 hours. The samples of the aged solution were pooled per concetration.
- Sample storage conditions before analysis: The samples were stabilized by acidification
and analyzed immediately after the test end.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of WAF according to OECD guidedance No. 23 and 29.
The test item was added to the dilution water and stirred for 24 hours at ca. 20 °C. Insoluble parts were separated from the aqueous phase by filtration (0.22 μm PES membrane). The obtained solution was used as the highest test concentration. All other test concentrations were obtained by dilution of the highest test concentration.The test solutions were freshly prepared before test start.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Clone V
- Source: German Federal Environment Agency, Institut für Wasser-, Boden und Lufthygiene. Specimens used in the test were bred in the laboratory of the Fraunhofer IME.
- Age at study initiation (mean and range, SD): 4 - 24 h
- Method of breeding: Batches of 30 - 50 specimen were held at room temperature in 1.8 L dilution water for one week and fed with a suspension of Desmodesmus subspicatus.
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

1.1 mmol/L

Test temperature:

20.6 – 20.8 °C

pH:

7.9 – 8.2

Dissolved oxygen:

8.4 - 8.9 mg/L

Nominal and measured concentrations:

Nominal substance concentration: 6.25, 12.5, 25, 50, and 100 mg/L
The nominal substance concentrations correspond to:0.72, 1.44, 2.89, 5.77 and 11.54 mg aluminium/L
Mean measured test substance concentrations: 0.0105, 0.00254, 0.0529, 0.1205, 0.2455 mg Al/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, covered with glass planes
- Material, size, fill volume: glass, 50 mL nominal volume, 50 mL fill volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water
- Alkalinity: 2.2 mmol/L
- Conductivity: 235 μS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night regime
- Light intensity: 779 – 885 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study:
- Results used to determine the conditions for the definitive study: No effects up to 100 mg/L (non-GLP)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.245 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: mg aluminium/L

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 0.245 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: mg aluminium/L

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.245 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

other: mg aluminium/L

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no

Results with reference substance (positive control):

Test with the reference substance were conducted in regular intervalls.
The latest test resulted in a 24 h EC50 value of 1.35 mg/L (95% CL: 1.20 – 1.53 mg/L)

Reported statistics and error estimates:

Statistical calculations were performed using the computer programme ToxRat®

Validity criteria fulfilled:

yes

Description of key information

EC50 (48 h) > 100 mg/L (nominal)
NOEC(48 h) ≥ 100 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of aluminium metaphosphate (CAS 13776-88-0) to invertebrates was investigated in a study by Simon (2014). This test was conducted according to OECD 202 under GLP conditions. Test organisms (Daphnia magna) were exposed to the test substance for 48 hours, applying a static water regime. Since the test item has also a low water solubility, the test media were prepared using the Water Accommodated Faction (WAF) approach according to the OECD guidance documents No. 23 and 29 to obtain the maximal solubility of the test item under the test conditions. Analytical determinations were performed to ensure a proper maintenance of the test concentration throughout the experiment. No effects on immobilization or fitness of Daphnia magna exposed to the test material were reported and therefore, an EC50 above 100 mg/L (> 0.245 mg/L measured aluminium) and a NOEC ≥100 mg/L (≥ 0.245 mg/L measured aluminium) were determined.

Thus the substance is considered not acutely toxic to aquatic invertebrates.