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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994 - 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-[4-(aminocarbonyl)phenyl]-4-methoxy-3-nitrobenzamide
EC Number:
298-790-7
EC Name:
N-[4-(aminocarbonyl)phenyl]-4-methoxy-3-nitrobenzamide
Cas Number:
93839-20-4
Molecular formula:
C15H13N3O5
IUPAC Name:
N-(4-carbamoylphenyl)-4-methoxy-3-nitrobenzamide
Details on test material:
purity: 95 +- 1 % contains residual water

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Aktivated sludge from the predominantly domestic waste water treatment plant Frankfurt-Sindlingen; not adapted to the test substance and the reference substance

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Test temperature:
19.2 - 20.6 °C
pH:
7.0 - 7.1
Nominal and measured concentrations:
nominal concentrations : 500 mg/L and 1000 mg/L
Details on test conditions:
480 ml glass bottles (diameter 60 mm, height 170 mm), placed in a thermostated water bath.
Measuring the consumption rate of oxygen with an oximeter WTW Oxi 537.
Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: respiration inhibition
Results with reference substance (positive control):
The EC50 with the positive control (3,5-dichlorophenol) was between 5 mg/L and 30 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for toxicity to bacteria is greater 1000 mg/L
Executive summary:

The toxicity to bacteria of the test substance was determined according to OECD guideline 209. Activated not adapted sludge from the predominantly domestic waster water treatment plant Frankfurt-Sindlingen was used as inoculum. Nominal concentrations of 500 mg/L and 1000 mg/L were used in duplicate. The average value for the respiration inhibition was 7.4% (1000 mg/L) and 7.6% (500 mg/L).

3,5 -dichlorophenol was used as rferene substance in the concentrations 5, 20, 30 mg/L showing inhibition rates of 22.8, 66.9, 71.3%.