Registration Dossier
Registration Dossier
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EC number: 406-640-0 | CAS number: 136920-07-5 KEROFLUX ES 3241
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Directive XI/681/86/EEC, draft 4
- Principles of method if other than guideline:
- The chronic toxicity of the test substance to Daphnia magna was investigated in a 21 day semistatic test.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
- EC Number:
- 406-640-0
- EC Name:
- A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
- Cas Number:
- 136920-07-5
- Molecular formula:
- C58H114N4O6 - C154H308N6O4
- IUPAC Name:
- Reaction mass of 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C12-C18)alkylacetamide and {[2-(Carboxymethyl-di(C12-C18)alkylcarbamoylmethyl-amino)-ethyl]-di(C12-C18)alkylcarbamoylmethyl-amino}-acetic acid
- Reference substance name:
- Keroflux ES 3241
- IUPAC Name:
- Keroflux ES 3241
- Details on test material:
- Test Substance No.: 90/763
Batch No.: Technikumspartie 1, T 75492/ST 1414/90
Water solubility: < 1 mg/L
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration control analysis was conducted by extraction of the test substance with Freon (1,1,2-trifluorotrichloroethane) from the water samples.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test was performed with an aqueous extract (eluate) prepared with 10 mg/L. The test substance was reduced to small pieces by means of a mortar, an appropriate amount weighed out and suspended in test medium by stirring for 24 hours. Then, the suspension was filtered.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Age of the animals at the start of the test: 2-24 hours
- Age of stock animals: 2-4 weeks
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 18.9 - 21.3 °C
- pH:
- 7.7 - 8.2
- Dissolved oxygen:
- 7.7 - 8.9 mg/L
- Nominal and measured concentrations:
- Test concentration (nominal): 10 mg/L
- Details on test conditions:
- - Test vessels: Glass beakers, nominal volume 100 mL
- Test volume: 50 mL
- Number of animals / vessel: 1
- Total number of animals / concentration: 10
- Number of parallels / concentration: 10
- Light: Day/night rhythm 16/8
- Change of test solution: Each Monday, Wednesday and Friday
- Removal of young from beakers and counting: Daily
- Feeding: Daily, according to feeding schedule
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- other: LC0
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
