Registration Dossier
Registration Dossier
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EC number: 232-477-8 | CAS number: 8050-18-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008. Due to the low aqueous solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, 20 daphnids (4 replicates of 5 animals) were exposed to a Water Accommodated Fraction (WAF) of the test item at a nominal loading rate of 100 mg/L for 48 hours at a temperature of approximately 19 °C to 20 °C under semi-static test conditions.
Chemical analysis of the freshly prepared 100 mg/L loading rate WAF preparations at 0 and 24 hours showed measured test concentrations of 0.070 and 0.081 mg/L, respectively. Analysis of the corresponding aged preparations at 24 and 48 hours showed a decline in measured test concentrations to 0.027 and 0.043 mg/L, respectively.
Exposure of Daphnia magna to the test item gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate (NOELR) was 100 mg/L loading rate WAF.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
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