Hydrogen (glycinato-N,O)[sulphato(2-)-O,O']ferrate(1-)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

STRUCTURAL DETERMINANTS IN THE OXIDATION OF ALIPHATIC COMPOUNDS BY ACTIVATED SLUDGE

Author:

Malaney GW, Gerhold RM

Year:

1969

Bibliographic source:

Journal (Water Pollution Control Federation), vol. 41, no. 2, 1969, pp. R18–R33. www.jstor.org/stable/25036256.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test:
Determination of oxygen uptake/oxidation of the compound using the Warburg respirometer
- Short description of test conditions:
Exposure of individual chemical compounds at a concentration of 500 mg/L to activated sludge solids at 2,500 mg/L in the Warburg respirometer with oxygen uptake as the measure of oxidation of the compound. The theoretical oxygen demand (TOD) is defined as the concentration of oxygen in mg/L required to oxidize 500 mg/L of substrate completely, i.e., to CO2, water, nitrate, sulfate, and chlorate.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Glycine

EC Number:

200-272-2

EC Name:

Glycine

Cas Number:

56-40-6

Molecular formula:

C2H5NO2

IUPAC Name:

aminoacetic acid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

24 h

Initial test substance concentration

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

percent from TOD

Reference substance

Reference substance:

other: no

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Remarks:

percent of TOD

Value:

16.9

Sampling time:

24 h

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Interpretation of results:

readily biodegradable

Conclusions:

In the present study of Malaney and Gerhold the biological oxygen demand, i.e. the concentration of oxygen in mg/L required to oxidize 500 mg/L of substrate completely, of activated sludge incubated with glycine was determined. The result reveals that 16.9 % of the compound were degraded within 24 h, therefore glycine is less susceptible to oxidation than the other amino-acids investigated but considered readily biodegradable because it belongs to the same chemical family as the amino acids that are more susceptible to oxidation.

Executive summary:

In the study of Malaney (1969) the biological oxygen demand of activated sludge incubated with glycine was determined. Again, this study was conducted prior to implementation of standard guidelines and GLP. The study was reported in sufficient detail and provides evidence that glycine is readily biodegradable. The result reveals that 16.9 % of the compound were degraded within 24 h, therefore glycine is less susceptible to oxidation than the other amino-acids investigated but considered readily biodegradable because it belongs to the same chemical family as the amino acids that are more susceptible to oxidation.