L-Tyrosine, 3,5-dinitro-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identi-cal. The keeping is performed similar to the method described in the OECD guideline, fol-lowing SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.1 - 20.3 °C

pH:

7.0 - 7.7

Dissolved oxygen:

8.4 - 8.6 mg/L

Nominal and measured concentrations:

nominal concentration: 100 mg/L
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV -determination. The concentrations determined at the start of the test were 102 % of the nominal concentration. At the end of the test the deter-mined concentrations were 117 % of the nominal concentration. Therefore, the determi-nation of the biological results was based on the nominal concentration.

Details on test conditions:

Medium renewal twice a week
Photo period 16/8 hours, using neon tubes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

Based on the test results the NOEC, 48 h can be stated to be >= 100 mg/L and the EC50, 48 h can be stated to be > 100 mg/L, respectively.

Executive summary:

One valid experiment was performed.

The study was as a limit test at 100 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

None of the animals was immobilised in the blank control and the test solution.

Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingHPLC-UV-determination. The concentrations determined at the start of the test were 102 % of the nominal concentration. At the end of the test the determined concentrations were 117 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.

As no immobility could be observed no statistical evaluation was performed.

The following results were determined for the test itemL-Tyrosine, 3,5-dinitro- (wet product)(species:Daphnia magna).

48h-NOEC≥100 mg/L (nominal)
48h-LOEC> 100mg/L (nominal)
24h-EC₅₀> 100 mg/L(nominal)
48h-EC₅₀> 100 mg/L(nominal)

Description of key information

One study was performed according to OECD 202.

Based on the test results the NOEC, 48 h can be stated to be >= 100 mg/L and the EC50, 48 h can be stated to be > 100 mg/L, respectively.

For the classification according to CLP only the EC50 value is relevant. For the discussion of the classification see summary of aquatic toxicity.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L