2-Hydroxypropyl-β-cyclodextrine ethers

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

other: clay loam, sandy loam, loam

Test material

Test material information

Constituent 1

Reference substance name:

.beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose

IUPAC Name:

.beta.-Cyclodextrin, 2-hydroxypropyl cycloheptaamylose

Constituent 2

Reference substance name:

-

EC Number:

420-920-1

EC Name:

-

Cas Number:

128446-35-5

Molecular formula:

Hill formula: (C42H70-nO35)(C3H7O)n; n(mittel)=5,25

IUPAC Name:

5,10,15,25-tetrakis(hydroxymethyl)-40,44,47,49-tetrakis(2-hydroxypropoxy)-20,30,35-tris[(2-hydroxypropoxy)methyl]-2,4,7,9,12,14,17,19,22,24,27,29,32,34-tetradecaoxaoctacyclo[31.2.2.2³,⁶.2⁸,¹¹.2¹³,¹⁶.2¹⁸,²¹.2²³,²⁶.2²⁸,³¹]nonatetracontane-36,37,38,39,41,42,43,45,46,48-decol

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Physical Appearance: white powder
Batch No.: 01 (DRD#: SIETS 96.010)
Purity: 89.16%
Density: 991 kg/m³ (0.991 g/cm³) at 24.9 °C (SLI Report #97-4-6948)
Storage conditions: room temperature in a dark, ventilated cabinet

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
[*) Properties that should be provided for the HPLC method]
- Water solubility*: >= 180 g/L at 20 °C (SLI Report #97-10-7113)

Radiolabelling:

not specified

Study design

Test temperature:

20 ± 2 °C

HPLC method

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Micromass Quarrro I Triple Quadrupole Tandem Mass Spectrometer

MOBILE PHASES (50:50 Milli-Q water/acetonitrile (v/v))
- Type: isocratic

Batch equilibrium or other method

Analytical monitoring:

yes

Details on matrix:

Soils: Three soils, obtained from various agricultural sites, were used to determine the adsorption and desorption characteristics of hydroxypropylated .beta.-cyclodextrin. All three soils were classified as a clay loam (Mutchler clay loam), sandy loam (Mutchler sandy loam) and a loam (Wisconsin loam). The soils are hereafter referred to as clay loam, sandy loam and loam, respectively. Prior to testing, the soils were sieved through a 2-jJm screen to provide a uniform aggregate size and were stored at 17°C in plastic bags. A Sartorius Moisture Analyzer was used to determine the percent moisture present in the soil. An aliquot of the soil was placed on the balance and was dried using a programmed temperature of 150°C until a constant weight was obtained. Percent moisture was calculated using the difference between initial and final weights recorded by the balance. The moisture content of each soil was determined prior to each test in which it was used (i.e., preliminary or screening). All soil weights are reported on a dry weight basis.

The mean measured concentrations for test solutions fortified at 5.15 mg/L were both 5.1 mg/L. This is in very good agreement with the dosing concentration and indicates that the soil matrix did not cause a problem in the analysis of hydroxypropylated .beta.-cyclodextrin.

Details on test conditions:

TEST CONDITIONS
- Buffer: 10.3 µg/mL blank buffer solutions
- pH: 6.5-6.6
- other: The test containers used for this study consisted of 50-mL glass centrifuge tubes with Teflon®-lined caps. Individual test containers were identified by study number, soil type, concentration, replicate, matrix and phase of study. All test containers were shaken on
a Labline Orbital Shaker Model 3590 in an environmental chamber designed to maintain a constant temperature of approximately 20 ± 2 °C.

Results and discussion

Adsorption coefficient

Adsorption coefficient 1

Type:

other: mean K

Value:

2.5

Temp.:

20 °C

Remarks on result:

other: clay loam

Adsorption coefficient 2

Type:

other: mean K

Value:

0.71

Temp.:

20 °C

Remarks on result:

other: sandy loam

Adsorption coefficient 3

Type:

other: mean K

Value:

0.36

Temp.:

20 °C

Remarks on result:

other: loam

Adsorption coefficient 4

Type:

Koc

Value:

92

Temp.:

20 °C

Remarks on result:

other: clay loam

Adsorption coefficient 5

Type:

Koc

Value:

38

Temp.:

20 °C

Remarks on result:

other: sandy loam

Adsorption coefficient 6

Type:

Koc

Value:

20

Temp.:

20 °C

Remarks on result:

other: loam

Results: Batch equilibrium or other method

Recovery of test material:

91-102 %

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption/desorption test with hydroxypropylated .beta.-cyclodextrin was conducted in Mutchler clay loam (clay loam), Mutchler sandy loam (sandy loam) and Wisconsin loam (loam) soils using 0.01 M CaCl2 at a 5:1 solution to soil ratio. The results of this study indicate that hydroxypropylated .beta.-cyclodextrin is not bound to organic matter and is therefore considered highly mobile in Mutchler clay loam, Mutchler sandy loam and Wisconsin loam soils.

Executive summary:

The adsorption/desorption test with hydroxypropylated .beta.-cyclodextrin was conducted in Mutchler clay loam (clay loam), Mutchler sandy loam (sandy loam) and Wisconsin loam (loam) soils using 0.01 M CaCl2 at a 5:1 solution to soil ratio. The results of this study indicate that hydroxypropylated .beta.-cyclodextrin is not bound to organic matter and is therefore considered highly mobile in Mutchler clay loam, Mutchler sandy loam and Wisconsin loam soils.