Tetrasodium tripotassium (.my.-((2,2'-((3-imino-6-sulfonatophenyl-(N-methyl)-imino)bis((6-chloro-1,3,5-triazin-4,2-diyl)imino(2-hydroxy-5-sulfonato-3,1-phenylen)azo(phenylmethylen)azo))bis(4-sulfonatobenzoato))-dicuprate(7-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental phase: 16 Nov - 20 Nov 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 50, 100 mg/L- Concentrations:
- Sample storage conditions before analysis: refrigerator

Vehicle:

no

Details on test solutions:

All test solutions were prepared separately for each concentration by diluting 0.5 and 1.0 g test substance in test water with an ultra-turrax stirrer.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: Zierfisch-Center Kloeckner, Ludwigshafen, Germany
- Age at study initiation: ca. 3 months (hatching date: 10 Aug 1998)
- Length at study initiation: 3.6 cm mean
- Weight at study initiation: 0.33 g mean

ACCLIMATION
- Acclimation period: ca. 1 month
- Type and amount of food during acclimation: TetraMin and artemia ad lib.
- Feeding frequency during acclimation: workdays
- Health during acclimation (any mortality observed): 0% mortality within 7 d prior to test

FEEDING DURING TEST : no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

250 mg/L (CaCO3 in dilution water)

Test temperature:

22°C

pH:

8.3 - 8.4

Dissolved oxygen:

8.4 - 8.7 mg/L

Salinity:

N/A

Conductivity:

N/A

Nominal and measured concentrations:

Nominal: 0 (control), 50, 100 mg/L
Measured (arithmetic mean 1 h, 96 h): <1 (control), 49.1, 98.6 mg/L
Recovery: >97% in both concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, headspace, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water supply
- Chlorine: chlorine-free
- Ca/Mg ratio: 9:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h

EFFECT PARAMETERS MEASURED: mortality and clinical symptoms at 1, 24, 48, 72 and 96 h

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality or any clinical symptoms observed in control, 50 and 100 mg/L

Results with reference substance (positive control):

N/A

Reported statistics and error estimates:

N/A

Validity criteria fulfilled:

yes

Conclusions:

The test item did not cause any mortality or clinical symptoms in fish up to 100 mg/L (nominal, analytically verified). The 96-h LC50 to Danio rerio is >100 mg/L.

Description of key information

The test item did not cause any mortality or clinical symptoms in fish up to 100 mg/L (nominal, analytically verified). The 96-h LC50 to Danio rerio is >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The substance is not acutely harmful to fish.