Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 480-240-4 | CAS number: 185257-07-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material: Propanenitrile, 2-[bis(cyanomethyl)amino]
- Molecular formula: C7H8N4
- Molecular weight: 148.17
- Impurities: < 0.1 g/100 g water, 0.08 g/100 g nitrilotriacetonitrile, free hydrocyanic acid 14.5 mg/kg
- Purity: 99.9g/100g %
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Municipal activated sludge from the wastewater treatment plant of Mannheim/Baden Württemberg (Germany). The inoculum was collected on 19 June 2006 from the aeration tank of the plant and aerated in the Iaboratory until use. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size of about 1 mm. After settling the supernatant was discarded and the sludge suspension was filled up with tap water. This suspension was pre-aerated for about 24 hours at room temperature. The sludge of the suspension was adjusted to a concentration of 6.0 gIL dry weight and then added to the test vesseis to obtain a sludge concentration of 30 mg/L dry substance. At last the activated sludge suspension in the test vesseis was pre-aerated once again for about 24 hours at a temperature of 22±2 °C.
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10 - 20
- Sampling time:
- 28 d
- Details on results:
- Degradation in the inhibition control after 14 days: 30 %
BOD5 / COD results
- Results with reference substance:
- Degradation of reference substance after 14 days: 82 %
Applicant's summary and conclusion
- Interpretation of results:
- other: poorly biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
