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EC number: 947-763-4
CAS number: -
A study was conducted to determine the in
vitro skin corrosion potential of the test substance, 'mono- and di- C16
-18 PSE and C16-18 AE20 PSE' using reconstructed human epidermis (RHE),
according to a method similar to OECD Guideline 431, in compliance with
GLP. Three replicates of 25 µg of the test substance (with 25 µL of
distilled water), 50 µL of the negative control (water) and 8N positive
control substance (8N Potassium hydroxide) were added to millicells
MatTek Epiderm tissue samples for 3 minutes and 1 h time period. After
exposure periods, the tissues were rinsed with phosphate buffered saline
(PBS) to remove any residual material. After all tisses had been rinsed
and dosed, they were placed for MTT assay. The MTT plates were then
incubated for 3 h (at 37 ºC, 5% C02). After incubation period, the
optical density (OD) was determined by using the MTT reduction assay and
expressed as relative percentage of viability of the negative
control-treated tissues. The mean viability of cells exposed to the test
substance were 101% and 99% of the negative control after 3 min and 1 h,
respectively. The test substance did not reduce the viability to 50% or
below and should be considered as non-corrosive to the skin. Under the
study conditions, the test substance tested was determined to be
non-corrosive to the skin (CPT, 2003).
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