Reaction mass of 1,5-Naphthalenedisulfonic acid, 3,3'-[(4-methyl-1,2-phenylene)bis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino[2-(acetylamino)-5-methoxy-4,1-phenylene]-2,1-diazenediyl]]bis-, sodium salt (1:4) and 1,5-Naphthalenedisulfonic acid, 3,3'-[(3-methyl-1,2-phenylene)bis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino[2-(acetylamino)-5-methoxy-4,1-phenylene]-2,1-diazenediyl]]bis-, sodium salt (1:4)

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From December 13, 1988 to December 15, 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At start and finish of the test

Test solutions

Vehicle:

no

Details on test solutions:

1 L test solution was prepared by the addition of 111 mg of test substance in dilution water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Continuous laboratory cultures.
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test: No
- Food type: algae (Chlorella vulgaris)
- Photoperiod: 16 h light adn 8 h dark

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

CaCO3 256 mg/L

Test temperature:

20±1˚C

pH:

8.26 to 8.58

Dissolved oxygen:

8.9 to 9.4 mg/L

Nominal and measured concentrations:

Nominal concentration 111 mg/L and measured concentration at 0 h 111 mg/L (100% of the nominal) and at 48 h 109.7 mg/L (99% of nominal)

Details on test conditions:

Borosilicate glass beakers were used as test vessels, wuth four replicates per exposure concentration each containing 200 mL test solution. When the test solution were at test temperature 5 daphnids were added to each vessel in < 2 mLof dilution water giving total of 20 daphnids per concentration.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 48-h EC50 to Daphnia magna was therefore > 100 mg/l (nominal), equivalent to a mean measured concentration of 110.4 mg/L. The substance is not acutely harmful to aquatic invertebrates.

Executive summary:

A study was conducted to determine the short-term toxicity of the test substance to Daphnia magna according to OECD Guideline 202. Twenty daphnids were exposed to a nominal concentration of 100 mg/L for 48 h under static conditions. Analytical dose verification was conducted using spectrophotometry. Test substance concentrations were considered stable over the duration of the test. No immobilised animal was reported in the control and test substance treated groups. Under the study conditions, the 48 h EC50 in Daphnia magna was therefore > 100 mg/l (nominal), equivalent to a mean measured concentration of 110.4 mg/L (Brown, 1988).