Absolute of Boswellia Carterii (Burseraceae) obtained from exudate by organic solvents treatment and subsequent ethanol extraction

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06-12 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 13 and 14 October 2014/ signed on 8 april 2015

Type of method:

method according to Siwoloboff

Key result

Boiling pt.:

181.9 °C

Atm. press.:

>= 99 636 - <= 99 721 Pa

Decomposition:

yes

Decomp. temp.:

>= 137.7 °C

Findings:

Pre-test:

The test tube with test item was set into the aluminium block at 18.1 °C. The aluminium block was heated with gas burner.

The following observations were noted:

Observations	Temperatures
Shrivelled together	Since 52 °C
Completely melted to a yellow clear sticky mass	70.1 °C
On surface some bubbles with lots of fine foam.The foam vanish slowly.	Since 108 °C
Colourless condensate visible	Since 128 °C
Individual bubble	Since 130 °C
No foam anymore	Since 135 °C
Bubble chain, gas burner switched off	185 °C
No bubbles anymore	161.8 °C

After cooling down the test item appeared as a yellow-brown clear solid.

Main test:

Observations	Temperatures
	First Determination atmospheric pressure 99636 Pa	Second Determination atmospheric pressure 99721 Pa
Test start: Test item melted immediately to a clear yellow sticky mass with lots of fine foam, the sample became a liquid.	140.0 °C	136.0 °C
No foam anymore, individual bubble and colourless condensate visible, colour getting slightly darker	Since 145 °C	Since 137.7 °C
Boiling chain, gas burner switched off	223.6 °C	228 °C
Boiling chain broke	182.0 °C	181.7 °C

After cooling down the test item appeared as a yellow-brown clear solid.

No calculation following Sydney-Young was carried out as signs of decomposition (colourless condensate) were considered.

Results:

	Boiling point under decomposition	Pressure in Pa
First determination	182.0 °C (455.2 K)	99636 Pa
Second determination	181.7 °C (454.9 K)	99721 Pa
Mean	181.9 °C (455.0 K)

Mean ± standard deviation (without correction following Sydney Young)= 181.9 ± 0.2 °C (455.0 ± 0.2 K)

Conclusions:

The determination of the boiling point of test item was performed following the Siwoloboff Method.
First signs of possibly decomposition indicated by a colourless condensate and colour change were observed since 137.7°C (lowest value).
Therefore the boiling point of test item should be stated as: 181.9 ± 0.2 °C (455.0 ± 0.2 K) under decomposition of the test item.
This value is the mean of two independent determinations without Sydney-Young correction.

Executive summary:

The study was performed in order to determine the boiling point of test item according to OECD 103, resp. EU A.2, guideline, using the Siwoloboff method.

First signs of possibly decomposition indicated by a colourless condensate and colour change were observed since 137.7°C (lowest value).

Therefore the boiling point of test item should be stated as: 181.9 ± 0.2 °C (455.0 ± 0.2 K) under decomposition of the test item.

Description of key information

The boiling point of test item should be stated as: 181.9 ± 0.2 °C (455.0 ± 0.2 K) under decomposition of the test item.

This value is the mean of two independent determinations without Sydney-Young correction.

Key value for chemical safety assessment

Boiling point at 101 325 Pa:

181.9 °C

Additional information

A fully reliable experimental study, conducted according to OECD 103/ EU A2 guideline is available.

It is considered as a key study, and the result is retained as key data.