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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
2007
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Study conducted according to OECD Guideline 301 C but with deviations: no detail on material and methods
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
yes
Remarks:
no detail on material and methods
Principles of method if other than guideline:
Not applicable
GLP compliance:
not specified
Specific details on test material used for the study:
No data
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Details on study design:
No data
Preliminary study:
No data
Test performance:
No data
Parameter:
% degradation (O2 consumption)
Value:
>= 90 - <= 95
Sampling time:
28 d
Details on results:
- Indirect analysis (BOD): 91, 90 and 95%
- Direct analysis (GC): 100, 100 and 100%
Results with reference substance:
No data

None

Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
Under the test conditions, alpha-pinene was readily biodegradable.
Executive summary:

In a ready biodegradation study performed according to OECD Guideline 301 C, alpha-pinene was tested at concentrations of 100 mg/L and the inoculum was activated sludge (30 mg/L). The degradation of the test material was assessed by the determination of the oxygen consumption.

At 100 mg/L test concentration, 90 -95% degradation (biochemical oxygen demand) was reached in 28 days.

 

Under the test conditions, alpha-pinene was readily biodegradable.

 

Description of key information

Based on a weight of evidence approach with data on constituents, the registered substance cannot be considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Individual data about the ready biodegradability of the main known constituents of the substance are provided in the table below:

Constituents CAS No % typical* % range in the NCS** Biodegradation results Reference
Beta-pinene 127-91-3 13.0 9 - 20 Not readily biodegradable QSAR, BioWin v4.10
Alpha-pinene 80-56-8 1.2 0 - 3 Readily biodegradable MITI, 2007

* % typical has been taken from Certificate of Analysis - batch 0517/1

** % ranges have been taken from the SIP of the substance (see section 1.2 "Composition")

According to the SIP of the substance, unknown constituents represent 75 to 91% of the NCS.

With data on individual constituents, it is therefore not possible to conclude on the ready biodegradability properties of the registered substance, because of a lack of data at the whole substance scale.

By default, the substance is considered not readily biodegradable.