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EC number: 220-599-4 | CAS number: 2832-30-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- unknown
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- publication
- Title:
- Frameshift Mutagenicity of Certain Naturally Occurring Phenolic Compounds in the 'Salmo11ella/Microsome' Test: Activation of Anthraquinone and Flavonol Glycosides by Gut Bacterial Enzymes
- Author:
- JOSEPH P. BROWN, PAUL S. DIETRICH and RONALD J. BROWN
- Year:
- 1 977
- Bibliographic source:
- Biochemical Society Transactions, 5(5), 1977, pp. 1489-1492
Materials and methods
- Principles of method if other than guideline:
- Study conducted according to principles of AMES test as described in Ames, B. N., Mccann, J. & Yamasaki, E. (1975) Mutat, Res. 31, 347-364.
Manipulation of cultures, preparation of crude microsomal fraction (S9) from Aroclor 1254-induced rats and criteria used in scoring results were was conducted according to Brown, J.P. & Brown, R. J. (1976) Murat, Res. 40, 203-224. - GLP compliance:
- no
- Remarks:
- Study conducted prior to implementation of GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-dichloro-5,8-dihydroxyanthraquinone
- EC Number:
- 220-599-4
- EC Name:
- 1,4-dichloro-5,8-dihydroxyanthraquinone
- Cas Number:
- 2832-30-6
- Molecular formula:
- C14H6Cl2O4
- IUPAC Name:
- 1,4-dichloro-5,8-dihydroxy-9,10-dihydroanthracene-9,10-dione
Constituent 1
Method
- Target gene:
- Histidine locus
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA1537, TA1538, TA98, TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver microsomal S9 from Aroclor 1254-induced rats
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
Applicant's summary and conclusion
- Conclusions:
- 5,8-Dichlorchinizarin was evaluated in the bacterial reverse mutation assay (Ames test) using Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98 and TA100 in the presence and absence of rat liver S9 mix. Under the conditions of the study, the test substance was negative for mutagenic potential.
- Executive summary:
In a reverse gene mutation assay in bacteria according to Ames et al. (adopted 1975), Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98 and TA100 were exposed to 5,8-Dichlorchinizarin in concentrations of 10, 50, 100 and 500µg/plate in the presence and absence of mammalian metabolic activation (rat liver S9 mix).
Revertants/nmol, i.e. the increase in numbers of revertants/plate per nmol increase in the quantity of test agent over the linear portion of the dose-response curve was <0.01 in the test groups with and without metabolic activation.
Accordingly, no substantial increase in revertant colony numbers of any of the five tester strains was observed following treatment with 5,8-Dichlorchinizarin at any concentration level, neither in the presence nor absence of metabolic activation (S9).
There was no evidence of induced mutant colonies over background.
Under the conditions of the study, the test substance was negative for mutagenic potential.
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