Registration Dossier
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EC number: 228-327-6 | CAS number: 6227-20-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 October 2016 to 14 December 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge : Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK,
- Storage conditions: on continuous aeration at a temperature of approximately 21 ºC
- Storage length: used the day of collection
- Preparation of inoculum for exposure: washed twice by settlement and resuspended in mineral medium
- Pretreatment: none
- Concentration of sludge: ss 3.0 g/L
- Water filtered: yes
- Type and size of filter used: 0.45 µm Gelman AcroCap filter - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20.7 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 10 mg/L
- Based on:
- other: mg C/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Additional substrate: none
- Solubilising agent (type and concentration if used):none
- Test temperature: 22-24 °C
- pH: 7.7-7.8 pre-adjustment; 7.3-7.6 after adjustment
- Aeration of dilution water: CO2 free air at 30-100 mL/min
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5 L culture vessels containing 3 L solution
- Number of culture flasks/concentration: inoculum control, procedure control and test substance in duplicate; toxicity control (single)
- Method used to create aerobic conditions: purging of CO2 free air and stirred continuously by magnetic stirrer
- Measuring equipment: TOC analyzer (Shimadzu TOC-VCPH TOC Analyzer)
- Test performed in closed vessels
- Details of trap for CO2 and volatile organics if used: NaOH (2 traps)
SAMPLING
- Sampling frequency: Days 0, 2, 6, 8, 10, 14, 21, 28 and 29 from first absober: days 0 and 29 from second absorber
- Sample storage before analysis: analysed immediately
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: NA - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- test for potential adsorption to filter matrices and/or the activated sewage sludge. 200 mg/L test substance was assessed for DOC in presence and absence of sludge with or without filtration through 0.45 µm Gelman AcroCap filter (4 treatments).
DOC concentration was 80-86% of nominal. No effect of filtration and or presence of sludge. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 29 d
- Remarks on result:
- other: after acidification with HCl
- Details on results:
- see table
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not biodegradable under the conditions of the test.
- Executive summary:
In a CO2 evolution test (OECD 301B), the substance degradation was found to be 6% after 29 days. Therefore the substance is considered not readily biodegradable.
Reference
% Biodegradation
Day |
Procedure Control |
Test Item |
Toxicity Control |
0 |
0 |
0 |
0 |
2 |
47 |
2 |
25 |
6 |
63 |
4 |
37 |
8 |
64 |
8 |
39 |
10 |
66 |
8 |
42 |
14 |
70 |
11 |
43 |
21 |
63 |
12 |
41 |
28 |
62 |
6 |
41 |
29* |
72 |
6 |
39 |
*include any carry-over of CO2 detected in Absorber 2
Description of key information
In a CO2 evolution test (OECD 301B), the substance degradation was found to be 6% after 29 days. Therefore the substance is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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