3,7-bis(diethylamino)phenoxazin-5-ium acetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from Publication

Data source

Reference

Reference Type:

publication

Title:

Toxicologic Studies of Four Fluorescent Whitening Agents

Author:

Keplinger et al.

Year:

1974

Bibliographic source:

Toxicology and Applied Pharmacology

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as metioned below

Principles of method if other than guideline:

Acute dermal toxicity study of Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate (16090-02-1) in rabbits.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

EC Number:

240-245-2

EC Name:

Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

Cas Number:

16090-02-1

Molecular formula:

C40H40N12O8S2.2Na

IUPAC Name:

disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

Details on test material:

Name: Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate
Smiles -c1(N2CCOCC2)nc(Nc2cc(c(\C=C\c3c (cc(Nc4nc(N5CCOCC5)nc(n4)Nc4ccccc4)
cc3)S(=O)(=O)[O-])cc2)S(=O)(=O)[O-])nc(n1)Nc1ccccc1.[Na+].[Na+]
InChI - 1S/C40H40N12O8S2.2Na/c53-61(54,55)33-25-31(43-37-45-35(41-29-7-3-1-4-8-29)47-39(49-37)51-17-21-59-22-18-51)
15-13-27(33)11-12-28-14-16-32(26-34(28)62(56,57)58)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52-19-23-60-24-20-52;;
/h1-16,25-26H,17-24H2,(H,53,54,55)(H,56,57,58)(H2,41,43,45,47,49)(H2,42,44,46,48,50);;/q;2*+1/p-2/b12-11+;;
- Molecular formula (if other than submission substance): C40H38N12Na2O8S2
- Molecular weight (if other than submission substance): 924.928 g/mole
- Substance type: organic

Specific details on test material used for the study:

Name: Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate
Smiles -c1(N2CCOCC2)nc(Nc2cc(c(\C=C\c3c (cc(Nc4nc(N5CCOCC5)nc(n4)Nc4ccccc4)
cc3)S(=O)(=O)[O-])cc2)S(=O)(=O)[O-])nc(n1)Nc1ccccc1.[Na+].[Na+]
InChI - 1S/C40H40N12O8S2.2Na/c53-61(54,55)33-25-31(43-37-45-35(41-29-7-3-1-4-8-29)47-39(49-37)51-17-21-59-22-18-51)
15-13-27(33)11-12-28-14-16-32(26-34(28)62(56,57)58)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52-19-23-60-24-20-52;;
/h1-16,25-26H,17-24H2,(H,53,54,55)(H,56,57,58)(H2,41,43,45,47,49)(H2,42,44,46,48,50);;/q;2*+1/p-2/b12-11+;;
- Molecular formula (if other than submission substance): C40H38N12Na2O8S2
- Molecular weight (if other than submission substance): 924.928 g/mole
- Substance type: organic

Test animals

Species:

rabbit

Strain:

other: Albino rabbit

Sex:

not specified

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Type of coverage:

other: Dermal - intact and abraded sites

Vehicle:

water

Details on dermal exposure:

TEST SITE
- Area of exposure: intact and abraded sites
REMOVAL OF TEST SUBSTANCE
- Washing (if done): unabsorbed test material was removed

Duration of exposure:

24 h

Doses:

5000 and 10000 mg/kg

No. of animals per sex per dose:

not specified

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: Animals were observed for clinical signs and gross pathology examination.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No Mortality observed

Mortality:

No Mortality observed at 10000 mg/kg

Clinical signs:

Moderate erythema disappearing by day 4, was observed; but there were no signs of systemic toxicity.

Body weight:

not specified

Gross pathology:

No gross pathologic changes were observed

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The LD50 was considered to be >10000 mg/kg bw, when albino rabbits were treated with Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate (16090-02-1) occlusively for 24 h exposure by dermal application to intact and abraded sites.

Executive summary:

Acute Dermal toxicity study was conducted in rabbits using Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate (16090-02-1) at the concentration of 5000 and 10000 mg/kg bw. Test material was applied to both intact and abraded sites on rabbits.After occlusion for 24 hr, unabsorbed test material was removed and treated sites were examined for local reactions. After an observation period of 14 days, the animals were sacrificed and examined for gross pathologic changes.No Mortality observed at 10000 mg/kg bw.Moderate erythema disappearing by day 4, was observed; but there were no signs of systemic toxicity.No gross pathologic changes were observed. Therefore, LD50 was considered to be >10000 mg/kg bw, when albino rabbits were treated with Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate occlusively for 24 h exposure by dermal application to intact and abraded sites.