Registration Dossier
Registration Dossier
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EC number: 612-975-5 | CAS number: 6225-08-7
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
After 7 and 14 days of exposure to Genagen PA, no significant inhibition of the microbial nitrate transformation was observed for the test item concentrations 62.5 and 125 mg/kg soil dry weight. For the test item concentrations 250, 500 and 1000 mg/kg soil dry weight, negative nitrate formation rate were determined, due to low nitrate contents.
After 28 days of exposure, no significant inhibition of the microbial nitrate transformation was observed for the test item concentrations 62.5, 125 and 250 mg/kg soil dry weight. For the test item concentrations 500 and 1000 mg/kg soil dry weight, negative nitrate formation rate were determined.
For calculation of the ECx-values, the inhibition was set as 100 % for concentrations with negative nitrate-N formation rates.
After 7 days of exposure the EC10-, EC25- and EC50-values for inhibition of the nitrate-N formation rate were 130, 137 and 151 mg/kg soil dry weight, respectively.
After 14 days of exposure the EC10-, EC25- and EC50-values for inhibition of the nitrate-N formation rate were 162, 173 and 192 mg/kg soil dry weight, respectively.
After 28 days of exposure the EC10-value for inhibition of the nitrate-N formation rate in soil was 268 mg/kg soil dry weight, the EC25-value was 284 mg/kg soil dry weight and the EC50-value was 311 mg/kg soil dry weight.
The corresponding no-observed effect concentration (NOEC) was 125 mg Genagen PA/ kg soil dry weight on day 7 and 14 and 250 mg Genagen PA/kg soil dry weight on day 28. The LOEC was determined to be 250 mg Genagen PA/kg soil dry weight after 7 and 14 days and 500 mg Genagen PA/ kg soil dry weight after 28 days of exposure.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 268 mg/kg soil dw
Additional information
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