2,2,4(or 2,4,4)-trimethylhexane-1,6-diamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-03-22 to 1983-03-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods with acceptable restrictions: Exposure period only 48 hours, incomplete documentation, no analytical monitoring

Principles of method if other than guideline:

Method: other: DIN 38412 part 15

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 10 g test substance/l

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISMS
- Strain: Leuciscus idus melanotus HECKEL
- Supplier: Eggers, Hohenwestedt
- Wild caught: no
- Age/size/weight/loading: 6 +/- 2 cm
- Feeding: daily 3 % of body weight TetraMin
- Pretreatment: single treatment with Zephirol 1:50,000 for 1 hour followed by 14 days under quarantine
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Test temperature:

20 +/- 1 degree C

pH:

8.2 - 10.1

Dissolved oxygen:

6.0 - 8.0 mg/l

Nominal and measured concentrations:

130; 160; 200; 250; 300 mg/l (nominal)

Details on test conditions:

DILUTION WATER
- Source: dechlorinated drinking water
- Aeration: continuous

TEST SYSTEM
- Exposure vessel type: 10 l solution in 18 l aquarium
- Number of replicates, fish per replicate: 1, 10
- Adjustment of pH: no
- Photoperiod: 16 hours light / 8 hours dark

TEST PARAMETER: mortality

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

130 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

174 mg/L

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

200 mg/L

Details on results:

No. of dead animals (48 hours): 
130 mg/l: 0 (0 %)
160 mg/l: 2 (20 %)
200 mg/l: 10 (100 %)
250 mg/l: 10 (100 %f)
300 mg/l: 10 (100 %f)

The LC50-value was determined graphically.

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (48 hours) for fish (Leuciscus idus melanotus) was determined as 174 mg/L indicating that the test substance does not pose a significant
hazard to fish.

Executive summary:

The acute toxicity of the test item on Leuciscus idus melanotus was evaluated in a static fish test over a period of 48 hours (Hüls AG 1996). The test was conducted according to national guideline DIN 38412, part 15. The following endpoint was derived:

LC50 (48 hours): 174 mg/L

The study was assessed as "reliable with restrictions".

Description of key information

The acute toxicity of the test item on Leuciscus idus melanotus was evaluated in a static fish test over a period of 48 hours (Hüls AG 1996). The test was conducted according to national guideline DIN 38412, part 15. The LC50 (48 hours) for fish (Leuciscus idus melanotus) was determined as 174 mg/L indicating that the test substance does not pose a significant hazard to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

174 mg/L