Exo-1-methyl-4-(1-methylethyl)-7-oxabicyclo[2.2.1]heptan-2-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-12-18 to 1987-12-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(1S,2S)-1-methyl-4-(propan-2-yl)-7-oxabicyclo[2.2.1]heptan-2-ol

Cas Number:

87172-89-2

IUPAC Name:

(1S,2S)-1-methyl-4-(propan-2-yl)-7-oxabicyclo[2.2.1]heptan-2-ol

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 53, 95, 171, 309, 556 and 1000 mg/L
- Sampling method: Samples of water (50-60 mL) were removed from each freshly prepared test solution (t0) and at 24 h after their preparation (t24).

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Fish were obtained from commercial hatcheries.

ACCLIMATION
- Acclimation period: Minimum of 7 days before the test begins

FEEDING DURING TEST: No

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

236 to 148 mg/L as CaCO3

Test temperature:

14.3 to 14.7

pH:

7.4 to 7.9

Dissolved oxygen:

8.4 to 10.2 mg/L

Salinity:

not applicable

Conductivity:

no data

Nominal and measured concentrations:

Nominal concentrations: 53, 95, 171, 309, 556 and 1000 mg/L
Mean measured concentrations: See table 3 in the section 'Any other information on results incl. tables'

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria, each filled with 10 litre of test solution
- Aeration: The aquaria were gently aerated to maintain the concentration of dissolved oxygen.
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The water used for maintenance of stock and for the toxicity test comes from two pumping stations (Newnham and Wychling) controlled by the Mid Kent Water Company. The water is obtained from bore holes in the chalk of the North Downs. The only chemical treatment prior to its arrival in the laboratory is chlorination to 0.1 mg/L. In the laboratory the water was filtered (Balstin LP-200-50-30) to remove particles larger than 10 µm and passed through activated carbon filters (Cano model CT) to remove chlorine and organic contaminants. Both particle and activated carbon filter cartridges are renewed as recommended by the manufacturers. Heat exchange units (stainless steel and perspex) were used to adjust the temperature of the water. Prior to use in the tests the water was virgorously aerated for several hours to ensure that no residual chlorine remained.
Samples of the water, after filtration but before aeration, are taken at approximately 6 month intervals and analysed for a number of parameters. These analyses are carried out by the Mid Kent Water Company.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: At 24 h intervals the fish were observed and the number of fish exhibiting toxic symptoms was recorded. The symptoms were classified into one of five categories from (a) non toxic to (e) dead. At each 24 h interval, any dead fish were removed.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1: Break-through concentration of the test item with Bond Elut C18 cartridges

Concentration of the test item added (mg/L) – 50 mL volume	Recovery (%)
200	89
300	83
400	63
500	55
1000	31

  

Table 2: Repeatability study of the test item analysis

Replicate number	% Recovery
	Concentration of the test item added (mg/L)
	10	100
(i)	89	96
(ii)	90	105
(iii)	96	99
(iv)	96	98
(v)	93	105

 

Table 3: Concentration of the test item in water – acute toxicity to Oncorhynchus mykiss

Nominal concentration of the test item in water (mg/L)	Determination concentration of the test item in water (mg/L)
	Day 1		Day 2		Day 3		Day 4		Mean t0 h	Mean t24 h
	0-24 h		24-48 h		48-72 h		72-96 h
	t0	t24	t0	t24	t0	t24	t0	t24
Control	<0.01	<0.01	<0.01	<0.01	0.01	<0.01	<0.01	<0.01	<0.01	<0.01
53	55	56	48	43	55	59	49	53	52	53
95	97	96	85	85	89	82	84	83	89	87
171	170	145	145	150	150	155	150	150	155	150
309	285	280	295	165	180*	280*	270	355	260	275
556	515	490	470	465	525	490	505	515	505	490
1000	980	1000	865	820	845	900	840	910	885	910

 

Recovery data

Concentration of the test item (mg/L)	Recovery (%)
100	93, 98, 93, 84, 94, 92, 94, 96

 

Table 4: Concentration of the test item in Oncorhynchus mykiss test solutions. Fresh solutions are expressed as a percentage of the nominal concentration. Old solutions are expressed as a percentage of the corresponding fresh solution.

Nominal concentration (mg/L)	Concentration of the test item in solution (%)
	t0	t24		t48		t72		t96
	fresh	old	Fresh	old	fresh	old	Fresh	old
Control	-	-	-	-	-	-	-	-
53	104	102	91	90	104	107	92	108
95	102	99	89	100	94	92	88	99
171	99	85	85	103	88	103	88	100
309	92	98	95	56	*	*	87	131
556	93	95	85	99	94	93	91	102
1000	98	102	87	95	85	107	84	108
range	92-104	85-102	85-95	56-103	85-104	92-107	84-92	99-131

*The concentration determined in the freshly prepared 309 mg/L nominal concentration test solution at t48 hours was abnormally low (58% of nominal). Since the concentration found in the same tank sample 24 h later was close to nominal (91%) this abnormality was considered to be possibly due to poor mixing prior to sampling for analysis, and the values have therefore been omitted from this table.

Table 5: Toxic symptoms exhibited by Oncorhynchus mykiss exposed to a range of concentrations of the test item

Concentration of the test item	Number of fish	Observation time and symptom classification*
		24 h					48 h					72 h					96 h
		a	b	c	d	e	a	b	c	d	e	a	b	c	d	e	a	b	c	d	e
Control	10	10					10					10					10
53	10	10					10					10					10
95	10	10					10					10					10
171	10	10					10					10					10
310	10	10					10					10					10
556	10	10					10					10					8		2
1000	10				8	2				7	3				6	4				6	4

 

Applicant's summary and conclusion

Conclusions:

In this 96h acute fish toxicity test, an EC50 >1000 mg/L was determined for the test item in rainbow trout (Salmo gairdneri).

Executive summary:

The acute toxicity of the test item to the rainbow trout, Oncorhynchus mykiss (previous name: Salmo gairdneri), was determined in a 96 h semi static toxicity test with renewal of the test solutions at 24 h intervals. Rainbow trout were exposed to the following test item concentrations: 53, 95, 171, 309, 556 and 1000 mg/L. At 96 h only 4 of the 10 fish exposed to 1000 mg/L, the highest concentration tested, were dead. The 96 h LC50 was therefore greater than 1000 mg/L. The concentrations of the test item in freshly prepared test solutions ranged from 84 to 104% of nominal. After 24 h in the test vessels concentrations of the test item in the test solutions ranged from 56 to 131% of the initial concentrations.