17-hydroxy-6-methylenepregn-4-ene-3,20-dione 17-acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17-hydroxy-6-methylenepregn-4-ene-3,20-dione 17-acetate

EC Number:

251-133-8

EC Name:

17-hydroxy-6-methylenepregn-4-ene-3,20-dione 17-acetate

Cas Number:

32634-95-0

Molecular formula:

C24H32O4

IUPAC Name:

17-Acetoxy-6-methylene-4-pregnene-3,20-dione

Details on test material:

- Name of test material (as cited in study report): 6-methylene-hydroxiprogestesteroneacetate
- Lot/batch No.: 877990

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Table 1: Cumulative immobilization (%)

No. of Daphnia	Test concentration	24 hours	48 hours
20	0,0 mg/L	1	1
20	0,1 -0,9 mg/L	1	1

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of 6-methylene-hydroxiprogestesteroneacetate to Daphnia magna is higher than the maximum solubility of the test compound under
the conditions of the test. It has to be noted that the test solution was not stable over the test period, i.e. the concentration decreased within
24 hours to approximately 20 % of the initial value .

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, ECs50/48 hours) of the product 6-methylene-hydroxiprogestesteroneacetate to Daphnia magna in a limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202.

Twenty Daphnia were used for each test concentration and for the control group. The Daphnia were exposed to the test concentration and the dilution water for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. According to the analysis, the test concentration was 0.9 mg/L at the beginning of the test and 0.1 mg/L at the end of the test (mean over 4 test vessels). One immobilized Daphnia was observed both in the test concentration and in the control group.