Disodium 4,4'-bis[(4-anilino-6-methoxy-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1979

Reference 2

Reference Type:

other: authoritative database

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Bioaccumulation study was conducted on test organism Pimephales promelas (fathead minnow) for 32 days for evaluating the bioconcentration factor (BCF value) of test chemical Disodium 4,4'-bis[[6-anilino-4-[(2-hydroxyethyl)methylamino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Test material form:

solid

Details on test material:

- Name of test material (IUPAC Name): Disodium 4,4'-bis[[6-anilino-4-[(2-hydroxyethyl)methylamino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- Common name: Tinopal 5BM
- Molecular formula: C38H38N12Na2O8S2
- Molecular weight: 900.9062 g/mol
- Smiles notation: c1(c(\C=C\c2c(cc(Nc3nc(nc(n3)Nc3ccccc3)N(CCO)C)cc2)S(=O)(=O)[O-])ccc(c1)Nc1nc(nc(n1)Nc1ccccc1)N(CCO)C)S(=O)(=O)[O-].[Na+].[Na+]
- InChI: 1S/C38H40N12O8S2.2Na/c1-49(19-21-51)37-45-33(39-27-9-5-3-6-10-27)43-35(47-37)41-29-17-15-25(31(23-29)59(53,54)55)13-14-26-16-18-30(24-32(26)60(56,57)58)42-36-44-34(40-28-11-7-4-8-12-28)46-38(48-36)50(2)20-22-52;;/h3-18,23-24,51-52H,19-22H2,1-2H3,(H,53,54,55)(H,56,57,58)(H2,39,41,43,45,47)(H2,40,42,44,46,48);;/q;2*+1/p-2/b14-13+;;
- Substance type: Organic
- Physical state: Solid

Radiolabelling:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

32 d

Test conditions

Test temperature:

25⁰C

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquarium was used as a test vessel for the study.
- No. of organisms per vessel: 30

Reference substance (positive control):

not specified

Results and discussion

Bioaccumulation factor

Type:

BCF

Value:

< 2.1 dimensionless

Basis:

whole body w.w.

Time of plateau:

32 d

Remarks on result:

other: Other details not known

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF value) of test substance on Pimephales promelas (fathead minnow) was determined to be < 2.1 dimensionless, respectively.

Executive summary:

Bioaccumulation study was conducted on test organism Pimephales promelas (fathead minnow) for 32 days for evaluating the bioconcentration factor (BCF value) of test chemical.The study was performed at a temperature of 25⁰C.Water at 25 °C was intermittently dosed with the chemical at a nontoxic concentration in a flow-through aquarium. Total 30 minnows are placed in the aquarium, and composite samples of five fish are removed for analysis after 2, 4, 8, 16, 24, and 32 d of exposure.The bioconcentration factor (BCF value) of test substance on Pimephales promelas (fathead minnow) was determined to be < 2.1 dimensionless, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.