Reaction mass of ammonium dodecyl sulfate, ammonium tetradecyl sulfate and ammonium alkoxy-alkyl sulfate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 - 27 Nov 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Refer to the Analogue Approach Justification document provided in IUCLID6 Section 13.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sulfuric acid, mono-C12-18-alkyl esters, sodium salts

EC Number:

273-257-1

EC Name:

Sulfuric acid, mono-C12-18-alkyl esters, sodium salts

Cas Number:

68955-19-1

IUPAC Name:

sodium pentadecyl sulfate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all
- Sampling method: after 0, 24 , 48 hours
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Stock solution was prepared by dispersing 0.709 g of test material in 0 L of distilled water making a 200 mg/L stock. Test solutions were prepared by adding known volumes of this stock to 1 L of ASTM medium.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house culture
- Age at study initiation: female juveniles
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

164 +/- 5 mg/L [as CaCO3]

Test temperature:

20.2 +/- 0.2 °C

pH:

8.2 +/- 0.1

Dissolved oxygen:

8.0 +/- 0.5

Nominal and measured concentrations:

1-10 mg a.i./L
Mean concentrations were between 100 and 105% of nominal values. Effect concentration are therefore based on nominal concentrations.

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
ASTM medium

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hrs light/ 6 hrs dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED: mobility

TEST CONCENTRATIONS
- Test concentrations: 0, 1.0; 1.8; 3.2; 5.6; 10.0 mg a.i./L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: 2.3 - 3.3 mg/L

Details on results:

- Behavioural abnormalities: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Reported statistics and error estimates:

The EC50 was calculatied by the Probit analysis.

Any other information on results incl. tables

Cummulative percentage of immobile daphnids after 48 hours:

Nominal concentration (mg/L)	% immobile daphnids after 48 hours
0	0
1.0	0
1.8	15
3.2	65
5.6	95
10.0	100