Oils, fish, oxidized

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 20-OCT-2009 to 05-FEB-2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: the study was performed according to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Oils, fish, oxidized

EC Number:

269-122-1

EC Name:

Oils, fish, oxidized

Cas Number:

68187-75-7

Molecular formula:

Not necessary

IUPAC Name:

Triglycerides C12-24, even, saturated and unsaturated, oxidized

Details on test material:

- Name of test material (as cited in study report): Triglycerides, C12-24, saturated and unsaturated, oxidized- Substance type: UVCB- Physical state: liquid- Stability under test conditions: stable- Storage condition of test material: At room temperature, protected from light

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L- Sampling method: data not available- Sample storage conditions before analysis: data not available

Test solutions

Vehicle:

no

Details on test solutions:

The test item was tested for toxicity towards daphnia as aqueous extracts (“WAF”) from up to 100 mg test item/L (final concentration). For this purpose, different amounts of the test item were dispersed in dilution water. The elution of the water soluble part (WAF) of the test item took place on a shaking machine at 130 rpm speed over 24 h at room temperature, respectively. During that time an equilibration between the test item and the water was considered to be achieved. Thereafter, the suspensions were filtered through glass fibre filters in order to eliminate non-dissolved parts of the test item. The eluates were used for the test without further dilution steps.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: water flea- Strain: Daphnia magna STRAUS (clone 5)- Source: of a laboratory breed (derived from Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquee, France in 1978 and cultivated since then at BASF using medium M4 acc. Elendt) was used for the test. - Age at study initiation: not older than 24h- Method of breeding: the cultivation of the daphnias is performed in a way that the animals are transferred in new test medium (see below) at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Chlorella-cells.- Feeding during test: during the test the daphnia were not fedACCLIMATION: data not available

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

data not available

Test temperature:

between 19.1°C and 20.4°C

pH:

between 8.00 and 8.72

Dissolved oxygen:

control: 8.8 mg O2/L at T0 and 8.4 mg O2/L at T48100 mg/L: 5.0 mg O2/L at T0 and 8.2 mg O2/L at T48

Salinity:

not applicable (freshwater)

Nominal and measured concentrations:

nominal concentration: 0 and 100 mg/Lmeasured concentration: 0 and 20 mg/L at T0, 0 and 16 mg/L at T48

Details on test conditions:

TEST SYSTEM- Test vessel: 50 mL beakers made of glass- Type (delete if not applicable): data not available- Aeration: data not available- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4- Biomass loading rate: data not availableTEST MEDIUM / WATER PARAMETERS: test medium according Elendt M4 according to the guideline (prepared in ultra pure water)OTHER TEST CONDITIONS- Adjustment of pH: no- Photoperiod: 16h of light and 8h of darkness- Light intensity: data not availableEFFECT PARAMETERS MEASURED: after 24h and 48h the number of immobilised daphnia at each concentration of the test item is recordedTEST CONCENTRATIONS- Spacing factor for test concentrations: not applicable- Justification for using less concentrations than requested by guideline: limit test at loading rate of 100 mg/L- Range finding study- Test concentrations: 0, 1, 10 and 100 mg/L- Results used to determine the conditions for the definitive study: no immobilisation observed in the range finding test

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: no mortality observed

Results with reference substance (positive control):

In order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. The value of the EC50 (24h) in this period of time was 1.26 mg/L potassium dichromate. This value is in accordance with the recommendations given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test).

Reported statistics and error estimates:

not applicable

Any other information on results incl. tables

Table 1: result of the main test

Loading of the testitem(mg/L) (Eluates/“WAF“)		Ratio immobilised Daphnia [%]		pH-Value		O2-Content [mg O2/L]		Temperature [°C]
		24h	48h	t0	48h	t0	48h	t0	48h
Control (0)		0 [0/20]	0 [0/20]	8.72	8.14	8.8	8.4	19.9	19.1
100		0 [0/20]	0 [0/20]	8.35	8.00	5.0	8.2	20.4	19.1

Table 2: assumed values on basis of the nominal concentrations:

	t24h	t48h
NOEC	≥ 100 mg/L	≥ 100 mg/L
LOEC	> 100 mg/L	> 100 mg/L
EL10	> 100 mg/L	> 100 mg/L
EL20	> 100 mg/L	> 100 mg/L
EL50	> 100 mg/L	> 100 mg/L

The test results are based on the nominal concentrations being tested as the test item was measured to be stable at in the aqueous phase at ≥ 80 % during incubation.

Table 3: results of the analytical part

NominalConcen­tra­tion of the Test Item [mg/L]	mL extracted	E2924cm^-1	mg TS calculated (absolute)	mg TS/L net TS - Blank (mg/L)	mg TS/L net TS - Blank (mg/L) + Glass wall	Recovery on Basis of t0
Control, t0	500	0.0097	0,1	-
100, t0	500	0.9122	10.1	20.0
Control, t48h	415	0.0223	0.2	-	-	-
100, t48h	300	0.4503	5	16.0	16.2	81.0

TS =Test item

¹⁾LM = solvent trichlorotrifluoroethane

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

• In the control without any test item no immobilisation of the animals was observed. • In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (~5 mg O2/L).

Conclusions:

EL50 (48h) > 100 mg/LNOEC (48h) > 100 mg/L

Executive summary:

“Triglycerides, C12-24, even, saturated and unsaturated, oxidized“ was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to in­vestigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnias was observed at concentrations representing the water soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at concentrations up to 100 mg/L. As there was no effect towards the daphnias within the screening-test the main test was performed only as a limit test using the “WAF” from 100 mg/L of the test item as the sole test concentration. The following EL-values (effective loading rate) are assumed acordingly:

 

	t24h	t48h
NOEC	≥ 100 mg/L	≥ 100 mg/L
LOEC	> 100 mg/L	> 100 mg/L
EL10	> 100 mg/L	> 100 mg/L
EL20	> 100 mg/L	> 100 mg/L
EL50	> 100 mg/L	> 100 mg/L

 

The test results are based on the nominal concentrations being tested as the test item was measured to be stable in the aqueous phase at ≥ 80 % during incubation. The test is considered valid as all conditions for validity were met.