Reaction mass of Chromate(2-), [3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)] [N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthalenyl]]-, lithium sodium and Chromate(2-), [3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)][N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthalenyl]acetamidato(2-)]-, lithium sodium

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study following official guidelines, GLP compliant performed on a mixture containing the substance and other analogue substances

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

yes

Remarks:

14 days observation

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Mixture of polyvalent bacteria collected November 11,1993 in the aeration tank of a domestic sewage treatment plant, ARABasel CommunalBacteria concentration : 30 mg TS/I

Duration of test (contact time):

ca. 14 d

Initial conc.:

ca. 100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Water/Nutrient: as specified in the methodTemperature: 20 +/- 2°CTest duration: 14 daysLighting: none

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

48 h

Details on results:

BOD / ThOD = 0%

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test item was tested for ready biodegradation following OECD 301C. Under the test conditions the test item is not ready biodegradable.

Executive summary:

The ready biodegradability of the test item was determined in a 14 days MITI - Test (I) according to the OECD Guideline for Testing of Chemicals, No. 301C, Paris 1981 and the EEC-Directive, 79/831, Annex V, C.7. The test substance was tested in a concentration of 100 mg/l. The reference substance Natriumbenzoat was also tested in a concentration of 100 mg/l. Under the experimental conditions the test substance is not readily biodegradable.

Description of key information

not readily or inherently biodegradable

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The read-across substance was tested for ready biodegradation following OECD 301C. The read-across substance was tested for inherent biodegradation following OECD 302C Zahn Wellens test. Under the experimental conditions the test item is neither inherently nor readily biodegradable.

Based on the read-across considerations the same results apply to Acid Black 220.