14H-anthra[2,1,9-mna]thioxanthen-14-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

14H-anthra[2,1,9-mna]thioxanthen-14-one

EC Number:

240-385-4

EC Name:

14H-anthra[2,1,9-mna]thioxanthen-14-one

Cas Number:

16294-75-0

Molecular formula:

C23H12OS

IUPAC Name:

8-thiahexacyclo[10.10.2.0²,⁷.0⁹,²³.0¹³,¹⁸.0²⁰,²⁴]tetracosa-1(23),2,4,6,9,11,13,15,17,20(24),21-undecaen-19-one

Details on test material:

Purity: 97.8 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish, danio rerio,
- Strain: HAMILTON-BUCHANAN
- Source: Hoechst Marion Roussel Deutschland GmbH, ProTox
- Age at study initiation: approximately 6 weeks
- Length at study initiation: 2.8 - 3.4 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle (Germany)
- Feeding frequency: twice daily ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

2.3 mmol Ca(2+) + Mg(2+) /L

Test temperature:

21.3 - 21.4 °C

pH:

7.5 - 8.1

Dissolved oxygen:

6.8 - 9.2 mg O2 / L

Nominal and measured concentrations:

nominal: 100 mg/L

control: 0 mg/L

Details on test conditions:

The study was conducted in a static system. The test chambers, which were calibrated to 4 liters, were made of glass (length 20 cm, width 15 cm, height 20 cm) and stood in a water bath made of Hostalit Z® with a Plexiglas viewing panel. The temperature of the water bath was regulated by a
thermostat to 22 +- 1 °C. The chambers were illuminated from above from 06.00 a.m to 06.00 p.m. The light intensity directly
over the chambers was approximately 700 lux.
The test chambers were not aerated during the course of the study.
Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution.
Concentrations nominal: 0 and 100 mg/L
7 fish for each concentration

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of the substance is > 100 mg/L (based on nominal concentration) and is above the solubility limit of the test substance in the test water.

Executive summary:

The substance was tested for acute toxicity to the zebra fish according to OECD 203 guideline 203 over 96 hours in a static system.

Before insertion of the test organism the test concentration was stirred for 24 hours to ensure that the limit of solubility of the test substance in water was reached. The tested concentration showed a red turbidity. Particulate matters were observed on the water surface and the bottom of the test vessels.

The lethality values given are based on nominal concentrations.

The fish of the 100 mg/L group showed no effect in comparison with the negative control. In the control group no lethality was observed.

No lethality occurred up to the solubility limit of the test substance in the test water.

The LC50 value are therefore determined as > 100 mg/L (nominal).