Registration Dossier
Registration Dossier
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EC number: 291-759-9 | CAS number: 90480-27-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test material is considered to be readily biodegradable (OECD 301 B).
The test item attained 67 % biodegradation after 28 days. As a UVCB, the test item meets the criteria for rapid biodegradability (greater than 60 % within 28 days).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method was designed to be compatible with OECD Guidelines for Testing of Chemicals (1992) No 301B "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4 -C of Commission Regulation (EC) No 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph m).
The test item at a concentration of 10 mg carbon/L was exposed to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at approximately 21 °C for 28 days. The biodegradation of the test item was assessed by determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, and a toxicity control were used for validation purposes.
The test item attained 67 % biodegradation after 28 days and met the criteria for rapid biodegradation in a UVCB (greater than 60 % within 28 days). The toxicity control attained 65 % biodegradation after 14 days and 66 % biodegradation after 28 days thereby confirming that the test item did not exhibit an inhibitory effect on the sewage treatment micro-organisms used in the test. Sodium benzoate attained 99 % biodegradation after 14 days and 103 % biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions. Biodegradation values in excess of 100 % were considered to be due to sampling and analytical variation.
The test item attained 67 % biodegradation after 28 days. As a UVCB, the test item meets the criteria for rapid biodegradability (greater than 60 % within 28 days).
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