Reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study. However, assessment of biodegradability of multi-constituent substances has to be done by regarding the biodegradability of each constituent. Therefore, this study is used within a weight-of-evidence approach.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

445-630-0

EC Name:

-

Molecular formula:

Unspecified

IUPAC Name:

1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): Vinylglycoldiacetat, approx. 75 %
- Substance type: multi-constituent substance
- Physical state: liquid / colorless, clear

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from the wastewater treatment plant of the city Mannheim/Baden, Württemberg (Germany), collected at 28 March 2006 from the aeration tank of theplant and aerated in the laboratory until use.
- Preparation of inoculum for exposure: Inoculum was sieved with by a finely woven mesh (mesh size about 1 mm), washed with
tap water and pre-aerated for about 24 hours.
- Pretreatment: None
- Concentration of sludge: 4 mg/L dry substance.
- Initial cell/biomass concentration:
- Type and size of filter used, if any: woven mesh (mesh size about 1 mm)

Duration of test (contact time):

28 d

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 22 ± 2 °C.

TEST SYSTEM
- Culturing apparatus: 160 mL incubation bottles filled up to a volume of 100mL.
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: oxygen, available in the headspace of the bottles
- Measuring equipment: The carbon dioxide in the headspace is solved in the alkaline solution. The amount of Total Inorganic Carbon (TIC) was determined with a carbon analyzer.
- Test performed in closed vessels due to significant volatility of test substance: Yes
- Test performed in open system: No
- Details of trap for CO2 and volatile organics if used: carbon dioxide in the headspace is solved in the alkaline solution

SAMPLING
- Sampling frequency: every 4 days
- Sampling method: Total Inorganic Carbon (TIC) was determined with a carbon analyzer.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: No

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (inorg. C analysis)

Value:

> 70 - < 80

Sampling time:

28 d

Remarks on result:

other: 10-day window not met

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: The test substance is biodegradable.

Executive summary:

The test performed by BASF (2006) indicates the multi-constituent substance to be biodegradable. However, as the reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate is a multi-constituent substance, the assessment of the biodegradability of the mixture has to be applied by regarding the biodegradation behaviour of each constituent. Due to the results of testing or QSAR it can be assumed, that all components are at least biodegradable or readily biodegradable (according to OECD criteria). Only 3-Octylacetate, CAS-No. 4864-61-3 was calculated by use of QSAR to be moderately biodegradable (ca. 30% BOD/ThOD). With a median amount of 0,3%, this substance should be regarded as negligible for the overall assessment of the biodegradability of the multi-constituent substance.

Overall it can be stated, that the reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate is biodegradable.