Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-441-2 | CAS number: 615-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- The supporting QMRF report has been attached.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Prediction is done using OECD QSAR Toolbox version 3.3 with log Kow as the primary descriptor.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Pimephales promelas
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- No data
- Test temperature:
- 24 deg.C
- pH:
- 6.9
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- No data
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140.24 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
The prediction was based on dataset comprised from the following descriptors: EC50
Estimation method: Takes average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain
((((("a" or "b" or "c" or "d" or "e" ) and ("f" and ( not "g") ) ) and ("h" and ( not "i") ) ) and ("j" and ( not "k") ) ) and ("l" and "m" ) )
Domain logical expression index: "a"
Referential boundary: The target chemical should be classified as Anilines (Acute toxicity) by US-EPA New Chemical Categories
Domain logical expression index: "b"
Referential boundary: The target chemical should be classified as Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines AND SN1 AND SN1 >> Nucleophilic attack after metabolic nitrenium ion formation AND SN1 >> Nucleophilic attack after metabolic nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines by DNA binding by OASIS v.1.3
Domain logical expression index: "c"
Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Primary aromatic amine by DNA binding by OECD
Domain logical expression index: "d"
Referential boundary: The target chemical should be classified as Weak binder, NH2 group by Estrogen Receptor Binding
Domain logical expression index: "e"
Referential boundary: The target chemical should be classified as Anilines (amino-para) AND Anilines (Unhindered) by Aquatic toxicity classification by ECOSAR
Domain logical expression index: "f"
Referential boundary: The target chemical should be classified as Weak binder, NH2 group by Estrogen Receptor Binding
Domain logical expression index: "g"
Referential boundary: The target chemical should be classified as Moderate binder, NH2 group by Estrogen Receptor Binding
Domain logical expression index: "h"
Referential boundary: The target chemical should be classified as Anilines (Hemolytic anemia with methemoglobinemia) Rank A AND Anilines (Hepatotoxicity) Rank C by Repeated dose (HESS)
Domain logical expression index: "i"
Referential boundary: The target chemical should be classified as Chlorphentermine (Hepatotoxicity) Alert by Repeated dose (HESS)
Domain logical expression index: "j"
Referential boundary: The target chemical should be classified as Amine AND Aromatic compound AND Aryl chloride AND Aryl halide AND Halogen derivative AND Primary amine AND Primary aromatic amine by Organic functional groups, Norbert Haider (checkmol)
Domain logical expression index: "k"
Referential boundary: The target chemical should be classified as Alkyl halide by Organic functional groups, Norbert Haider (checkmol)
Domain logical expression index: "l"
Parametric boundary:The target chemical should have a value of log Kow which is >= 0.159
Domain logical expression index: "m"
Parametric boundary:The target chemical should have a value of log Kow which is <= 1.47
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The median effective concentration (EC50) for the test substance, 2-chloro-p-phenylenediamine, in Pimephales promelas on the basis of mortality effect was estimated to be 140.24 mg/L in a 96 hours of exposure.
- Executive summary:
A study was predicted using OECD QSAR toolbox version 3.3 (2017); to assess the toxicity effect of 2-chloro-p-phenylenediamine on fish in a 96 hours of exposure. The median effective concentration (EC50) for the test substance, 2-chloro-p-phenylenediamine, in Pimephales promelas on the basis of mortality effect was estimated to be 140.24 mg/L. Thus, on the basis of this EC50 value and according to CLP Criteria for aquatic classification of the substance, it is concluded that 2-chloro-p-phenylenediamine (CAS No. 615-66-7) does not exhibit short term toxicity to fish.
Reference
Description of key information
A study was predicted using OECD QSAR toolbox version 3.3 (2017); to assess the toxicity effect of 2-chloro-p-phenylenediamine on fish in a 96 hours of exposure. The median effective concentration (EC50) for the test substance, 2-chloro-p-phenylenediamine, in Pimephales promelas on the basis of mortality effect was estimated to be 140.24 mg/L. Thus, on the basis of this EC50 value and according to CLP Criteria for aquatic classification of the substance, it is concluded that 2-chloro-p-phenylenediamine (CAS No. 615-66-7) does not exhibit short term toxicity to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 140.24 mg/L
Additional information
Various studies including predicted results from the validated model and experimental study from a peer-reviewed journal for toxicity to fish with the target substance 2-chloro-p-phenylenediamine (CAS No. 615-66-7) and to its relevant read across substance 4-methyl-m-phenylenediamine (CAS No. 95-80-7) by considering its structure-activity relationships, were summarized as follows:
A study was predicted using OECD QSAR toolbox version 3.3 (2017); to assess the toxicity effect of 2-chloro-p-phenylenediamine on fish in a 96 hours of exposure. The median effective concentration (EC50) for the test substance, 2-chloro-p-phenylenediamine, in Pimephales promelas on the basis of mortality effect was estimated to be 140.24 mg/L.
In addition, based on the Ecological Structure Activity Relationships (ECOSAR) Predictive Model version 1.11 (2017), the median lethal concentration (LC50) for the test substance, 2-chloro-p-phenylenediamine, in fish was estimated to be 4337.28 mg/L on the basis of mortality effect in a 96 hour study.
Moreover, the study was designed by Holcombe,G.W. et al. (Arch. Environ. Contam. Toxicol.28(3): 287-297) to assess the toxic effects of the test compound 4-methyl-m-phenylenediamine on Oryzias latipes (Japanese Medaka) fish in a 96 hours of exposure. 50% mortality was observed. Thus, the median lethal concentration (LC50) for the test substance, 4-methyl-m-phenylenediamine, in Oryzias latipes on the basis of mortality effect was considered to be 912.0 (797 to 1040) mg/L.
Based on the above predicted and experimental studies for target substance 2-chloro-p-phenylene diamine (CAS No. 615-66-7) and to its read across substance 4-methyl-m-phenylenediamine (CAS No. 95-80-7), the effective concentration EC50 value was found to be 140.24 mg/L and LC50 value was found to be in the range of 912.0 to 4337.28 mg/L. Thus, on the basis of this EC50 and LC50 value and according to CLP Criteria for aquatic classification of the substance, it is concluded that 2-chloro-p-phenylenediamine (CAS No. 615-66-7) does not exhibit short term toxicity to fish.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.