Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate]

Endpoint summary

Description of key information

Short-term toxicity to fish

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on fish predicted for Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8). Lethal concentration i.e LC50 value estimated to be 116.15 mg/l for Danio rerio for 96 hrs duration. It can be concluded that the Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) likely to be not toxic to fish, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

Short-term toxicity to aquatic invertebrates

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment.

Toxicity to aquatic algae and cyanobacteria

The effect of test item dialuminium tris[2 -(2,4,5,7 -tetrabromo-6 -oxido-3 -oxoxanthen-9 -yl)benzoate], CAS No. 15876-39-8 was studied on the growth of fresh water green alga Chlorella vulgaris(UERL Study report, Sustainability Support Services (Europe) AB, 2016). The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 164.10 mg/L. Thus based on this value, it can be concluded that the substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

Toxicity to micro-organism

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to micro-organism predicted for Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8). Lethal concentration i.e IGC50 value estimated to be 22.62 mg/l for Tetrahymena pyriformis for 48 hrs duration.

Additional information

Short-term toxicity to fish

Following studies for target chemical and read across chemical includes results from OECD QSAR toolbox and experimental data from peer reviewed journals to conclude the Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) toxicity extent towards fish is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on fish predicted for Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8). Lethal concentration i.e LC50 value estimated to be 116.15 mg/l for Danio rerio for 96 hrs duration. It can be concluded that the Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) likely to be not toxic to fish.

The above predicted data is supported by the exprimental data of read across3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid (CAS: 18472-87-2)study from the peer reviewed journalSouthwestern Entomologist 1984, suggests that the

In 3,4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid, there was not mortality for the first 48h. However mortality was observed at 72 h and 96 hr exposure periods with LC50 values 120.4 and 97.7 respectively. It can be concluded from the value that the 3, 4,5,6-tetrachloro-2-(1,4,5,8-tetrabromo-6-hydroxy-3-oxoxanthen-9-yl)benzoic acid is not toxic to the aquatic environemt.

Further it is supported by the eprimental data of another read across Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate (CAS: 17372-87-1) from The Journal of Toxicological Sciences 1982, indicates that the Based on mortality of test fishes, the LC50 value was found to be 1,000 and 620 mg/l at 24 and 48 hrs, respectively.It can be concludede that the Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 116.15 mg/L mg/l to 1000 mg/l give the conclusion that test substance Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) was likely to be not toxic to fish at environmentally relevant concentrations and can be considered to be“not classified”as per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates

Following studyfromexperimental reports following standard testing procedures by ABITEC 2016, to conclude the toxicity extent of

Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) towards aquatic invertebrate is summarized as follows:

Short term toxicity to aquatic invertebrates was performed in Daphnia magna following the OECD guideline 202. The test was performed for 48 hrs at different concentrations as control(1,2,3), 10, 25, 50, 100,200 mg/l .Reference substance used is K2Cr207.

5 pieces of daphnids after breeding were used in 4 numbers of replicates of chemical.The test was perfromed at a temperature of 20°C±1°C with taking 25ml per replicate in 50ml glass vessel for 48 hrs. Calulations of EC50 was calculated using nonlinear regression by the software Prism4.0 (GraphPadSoftware,Inc.,SanDiegoCA).

The EC50 value for short term toxicity to Daphnia magna using OECD guideline 202 is 177.7 mg/l. It can be concluded from the value that the Dialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] is not toxic to the aquatic environment and can be considered as "not classified" as per CLP regulation.

Toxicity to aquatic algae and cyanobacteria

Following studiesfromexperimental reports following standard testing procedures by UERL and ABITEC 2016, to conclude the toxicity extent of

Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) towards aquatic invertebrate is summarized as follows:

Study from UERL 2016,indicates that The effect of test item dialuminium tris[2 -(2,4,5,7 -tetrabromo-6 -oxido-3 -oxoxanthen-9 -yl)benzoate], CAS No. 15876-39-8 was studied on the growth of fresh water green alga Chlorella vulgaris(UERL Study report, Sustainability Support Services (Europe) AB, 2016). The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 164.10 mg/L. Thus based on this value, it can be concluded that the substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

Similar studyfromexperimental reports following standard testing procedures by ABITEC 2016, suggests that ,A freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 15876-39-8 exerts different degrees of toxic effects on the growth of Desmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 27 hours of exposure. The nominal concentrations used for the exposure assessment were as follows: 5, 10, 20, 40 and 80 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical in Desmodesmus subspicatus.In conclusion, the EC50 for CAS 15876-39-8 was 72.6 mg/L (95% 61.6-85.5 mg/L) after 72 hours of exposure.

Thus theDialuminium tris[2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate]is not toxic to the aquatic environment can be considerd as "not classiified." as per CLP regulation.

Thus based on the effect concentrations which is in the range 72.6 mg/L mg/l to 164.10 mg/l give the conclusion that test substance Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) was likely to be not toxic to algae at environmentally relevant concentrations and can be considered to be“not classified”as per the CLP classification criteria.

Toxicity to micro-organism

Following studies for the target and read across chemical includes perdicted data from OECD QSAR toolbox and experimental data from peer reviewed journal to conclude the extent ofDi aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8) toxicity towards micro-oragnism as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to micro-organism predicted for Di aluminium tris [2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate] (CAS: 15876-39-8). Lethal concentration i.e IGC50 value estimated to be 22.62 mg/l for Tetrahymena pyriformis for 48 hrs duration.

The above predicted data is supported by the experimental data of read acrossdisodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate (CAS: 17372 -87-1) from ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Nov. 2002, suggests thatthe

Toxicity to micro-organisms study was conducted onStaphylococcus aureusof enterotoxigenic strain ATCC 13565.

 For the preparation of dye solution, a test portion (approximately 10 mg) was placed in a 10-ml volumetric flask, which was wrapped in aluminum foil, and dissolved by adding aqueous sodium hydroxide (approx. 0.4% NaOH) (1 ml) and water (9 ml).Inoculum was prepared inbrain heart infusion broth (BHI). Cultures were incubated shaking (200 rpm) at 37°C. All the experiments were conducted under conditions of standard room illumination (fluorescent ceiling light, Sylvania Octron 4100K) in tubes containing 3 ml of medium. Growth was analyzed by measuring turbidity (absorbance at a wavelength of 660 to 680 nm) with a spectrophotometer (Spectronic 21D). Experiments were initiated with  ̴0.2 ml of inoculum from a fresh culture grown aerobically at an optical density (OD) of ̴1. Colony counts were measured on BHI plates.

 For the study,a 300-µl aliquot of dye solution (1 mg/ml) was mixed with 0.7 ml of BHI and then added to a tube of diluted culture. The tubes were aerated vigorously, and the OD was measured every 45 min to check culture growth. The survival rate was measured by plating serial dilutions of a sample taken during the experiment on BHI plates.

 During the study period of 250 mins, OD was measured every 45 mins to check culture growth.Experiment was performed in triplicate on separate days to ascertain reproducibility.As the test substance do not have any activity againstS. aureusi.e, no difference was observed between the treated test tubes and the control tubes, the NOEC value was found to be 100 mg/l.

Thus, based on above all experimental studies for micro organisms, it can be concluded that the target chemical Eosine (CAS No. 17372-87-1) can be considered as non-toxic to micro organisms.