4,11-diamino-2-[3-(2-methoxyethoxy)propyl]-1H-naphth[2,3-f]isoindole-1,3,5,10(2H)-tetrone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the bath at the beginning and at the end of the experiment.

Vehicle:

not specified

Details on test solutions:

The test substance was disolved in 200 ml water, which was slowly added to the fish bath

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: west Aquarium, Bad Lauterberg, BRD
- Age at study initiation (mean and range, SD):109 days
- Length at study initiation (length definition, mean, range and SD): 30.2 mm
- Weight at study initiation (mean and range, SD): 0.28 g

ACCLIMATION
- Acclimation period: 26 days in dechlorinated tap water

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

249 mg/l CaCO3

Test temperature:

21.8- 24.3 °C

pH:

at start and each day

Dissolved oxygen:

at start and each day

Salinity:

No data

Nominal and measured concentrations:

The test concentrations were chosen as: 562 and 1000 ppm.
A control was carried out with the reconstituted water.

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 liter glass aquaria; 2.9 litre
(3.0 litre minus 2x 0.05 litre for analytical control)
- Aeration:slowly aerated

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 1 000 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No abnormal responses of the fish were observed.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

The LC50 after 96 h was >1000 ppm.

Executive summary:

The acute fish toxicity of FAT 36152/D was evaluated in accordance with the EEC Directive, Annex V, Part. C.1. corresponding to the OECD-Test guidelines No.203, (1984). In the pretest the LC50 was determinated to be >1000 ppm. The test concentrations were chosen as: 562 and 1000 ppm. A control was carried out with the reconstituted water. 10 fishes per concentration and water control were used in this static system. No abnormal responses of the fish were observed. Hence, based on the findings of the study, the LC50 after 96 h was found to be >1000 ppm.

Description of key information

The 96h LC50 of the target chemical was considered to be >1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

The potential of test substance to cause short term toxicity to fish was investigated in three different studies. The acute fish toxicity of FAT 36152/D was evaluated in accordance with the EEC Directive, Annex V, Part. C.1. corresponding to the OECD-Test guidelines No.203, (1984). In the pretest the LC5o was determinated to be >1000 ppm. The test concentrations were chosen as: 562 and 1000 ppm. A control was carried out with the reconstituted water. 10 fishes per concentration and water control were used in this static system. No abnormal responses of the fish were observed. Hence, based on the findings of the study, the LC 50 after 96 h was found to be >1000 ppm. This study was most recently conducted and considered as a key study. Short term toxicity of FAT 36152/B to Zebra fish was investigated in a study using the methodology equivalent to OECD Guideline 203. Groups each containing 10 zebra fishes were exposed for 96 h to the test substance at 100, 300 and 1000 mg/L. No mortality was observed with any of the test concentrations. Hence, the 96 h LC0, LC50 and LC100 to zebra fish (Danio rerio) were found to be greater than 1000 mg/L (1986).

 Short term toxicity of FAT 36152/A to fish was determined in a study conducted according to the methodology equivalent to OECD Guideline 203. Two groups of zebra fish, each containing 10 fishes, were exposed to test concentrations of 300 and 1000 mg/L for 96 hours. No mortality was observed with both concentrations. Hence, the 96 h LC0, LC50 and LC100 of zebra fish (Danio rerio) was considered to be greater than 1000 mg/L (1984).

No mortality or adverse effects were reported in the above discussed studies, hence the target chemical was considered to have low toxicity to fish with LC50 >1000 mg/L.