Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental test result performed using standard OECD test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra Fish (Danio rerio).Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate

EC Number:

222-656-9

EC Name:

Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate

Cas Number:

3567-66-6

Molecular formula:

C16H13N3O7S2.2Na

IUPAC Name:

disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate- Molecular formula (if other than submission substance): C16-H13-N3-O7-S2.2Na- Molecular weight (if other than submission substance): 467.3889 g/mol- Substance type: Organic - Physical state: Reddish brown powder- Analytical purity: 93.54% (Pure dye content)- Lot/batch No.: FG/15-16/2641- Expiration date of the lot/batch: December, 2023-Storage Conditions-•Ambient Temp (23 to 27 °C)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio - Source:Unique Aqua Fish Corner- Age at study initiation (mean and range, SD): No data available.- Length at study initiation (length definition, mean, range and SD):3.23 cm - Weight at study initiation (mean and range, SD):0.203 g (approx.)0.322 g- Feeding during test: No - Food type:Standard brand feed,- Frequency: once dailyACCLIMATION- Acclimation period: 6 days - Acclimation conditions (same as test or not):yes - Type and amount of food:Standard brand feed,- Feeding frequency:once daily up to sixth day of acclimatization- Health during acclimation (any mortality observed): No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

147.5

Test temperature:

25°C

pH:

7.01

Dissolved oxygen:

7.06 mg/L

Salinity:

No data available.

Nominal and measured concentrations:

100 mg/L.

Details on test conditions:

TEST SYSTEM- Test vessel: The test vessels used were all plastic aquaria having the water capacity of 5liters.- Type (delete if not applicable): open / closed- Material, size, headspace, fill volume:- Aeration:Aeration in test vessels was provided 1 day before the start of experiment- Type of flow-through (e.g. peristaltic or proportional diluter):- Renewal rate of test solution (frequency/flow rate):- No. of organisms per vessel: 4- No. of vessels per concentration (replicates):2- No. of vessels per control (replicates):1TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.OTHER TEST CONDITIONS- Adjustment of pH:- Photoperiod: 12 hours light and 12 hours darkness- Light intensity:

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality and symptoms

 

NominalConcentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h			48h		72h		96h
			V1	V2	V1	V2	V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-

 

Visible Symptoms: 

NominalConcentration (mg/l)	Number of fish		Symptoms after
			3 h		6 h		24h			48h		72h		96h
			V1	V2	V1	V2	V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-

 

Explanation of symptoms:

A:apathy

N:Narcotic-like state

C:Swimming near water surface

M: Slow movements compared to control

NS:No abnormal symptoms 

S:Surfacing behaviour

L:Loss of equilibrium

SM:Shimming movement

H1:Hyperactivity

H2:Hypo activity

B:Bending behavior

F:Fin rot

IS:Invert Swimming

SB:Swimming behaviour

RF:Respiratory Function

P:Pigmentation

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 3567-66-6 on Zebra Fish were found to be>100mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for __________. The nominal concentration selected for the experiment were ____________ and Zebra fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.