Benzyltriethylammonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08.11.2016 - 10.11.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Short term toxicity of test chemical were determine. Test conducted as per the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

No data

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION: The stock solution 200.0 mg/L was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted test water. The substance was declared as light sensitive.- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM - Common name: Water flea- Strain/clone: Straus - Source: Own breeding at University of Chemistry and Technology, Prague - Age: Young daphnids, aged less than 24 hours used at the start of the test and they should not be first brood progeny. - Feeding during test : Without feeding ACCLIMATION - Acclimation period: No data - Acclimation conditions (same as test or not): No data - Type and amount of food: No data - Feeding frequency: No data - Health during acclimation (any mortality observed): No data - QUARANTINE (wild caught) - Duration: No data - Health/mortality: No data

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

20 °C ± 1 °C

pH:

Sample at concentration 200 mg/L : pH = 7.7 did not change during the test, Control: pH = 7.8 changed to pH = 7.7 during the test.

Dissolved oxygen:

Higher than 7.9 mg/L both in the control and the sample

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

0, 6.24, 12.5, 25 , 50, 100 and 200 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: 50 ml glass vessel- Material, size, headspace, fill volume: 25 ml - Aeration: no data- No. of organisms per vessel: 5 pieces- No. of vessels per concentration (replicates): 4 - No. of vessels per control (replicates): No data - No. of vessels per vehicle control (replicates): No data - Biomass loading rate: No data TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l. OTHER TEST CONDITIONS - Adjustment of pH: Without adjustment - Photoperiod: No - Darkness EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 was calculated using nonlinear regression by the software Prism 4.0.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

31.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL : 21.2 – 47.9 mg/L

Details on results:

No data

Results with reference substance (positive control):

- Results with reference substance valid- 24h EC50 = 0.79 mg/L K2Cr2O7

Reported statistics and error estimates:

No data

Control solution:Reconstituted water without the sample , 0 % of the daphnids were immobilized at the end of the test.

Concentration of test substance:spectrophotometric determination at 209 nm after centrifugation of the sample (1395 g, 5 min) – start of the test 106.4 mg/L , the end of the test 96.6 mg/L.

 

c [mg/L]	Measured concentration at start of the test [mg/L]	Measured concentration at the end of the test [mg/L]
6.24	6.9	6.2
200	185.6	169.7

Validity criteria fulfilled:

yes

Conclusions:

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 31.9 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Executive summary:

Determination of the inhibition of the mobility of daphnids was carried out with the substance test material according to OECD Guideline 202.

The test substance was tested at the concentrations 0, 6.24, 12.5, 25, 50, 100 and 200 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 200.0 mg/L was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted test water. The substance was declared as light sensitive.- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution.The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 31.9 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that test substance exhibits toxicity to aquatic invertebrate (Daphnia magna) in the aquatic acute 3 classification category.

Description of key information

Short term toxicity to aquatic invertebrate:

Determination of the inhibition of the mobility of daphnids was carried out with the substance test materialaccording to OECD Guideline 202.

The test substance was tested at the concentrations 0, 6.24, 12.5, 25, 50, 100 and 200 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 200.0 mg/L was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted test water. The substance was declared as light sensitive.- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution.The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 31.9 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that test substanceexhibits toxicity to aquatic invertebrate (Daphnia magna) in the aquatic acute 3 classification category.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

31.9 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Experimental key and supporting study for the target chemical were reviewed to summarize the following information:

 

In key study, determination of the inhibition of the mobility of daphnids was carried out with the substance test materialaccording to OECD Guideline 202.

The test substance was tested at the concentrations 0, 6.24, 12.5, 25, 50, 100 and 200 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 200.0 mg/L was prepared by dissolving white powder in reconstituted water. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted test water. The substance was declared as light sensitive.- Controls: Reconstituted water as specified in OECD 202, used as a control and for sample dilution.The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 31.9 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that test substanceexhibits toxicity to aquatic invertebrate (Daphnia magna) in the aquatic acute 3 classification category.

 

Acute toxicity to aquatic invertebrate test from authoritative database has been carried out to evaluate the ecotoxicological effects of chemical with dose concentration of 8.0 mg/L, 17 mg/L, 38 mg/L, 83 mg/L and 180 mg/L.The test organism was Daphnia magna and the test carried out in static condition throughout the test, temperature maintained at 20 +/- 1 degree C. The 48-h EC50 value was observed to be 63.1 mg/L when exposed to Daphnia magna with test chemical .

  

Considering the above key and supporting data, it can be concluded that the substance exhibits toxicity to aquatic invertebrate (Daphnia magna) in the chronic 3 classification category as per CLP classification criteria.