1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (6E)-, hydrogenated

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive and done to a valid guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(6E)-2,6-dimethyl-10-methylidenedodeca-2,6-diene; (6E)-3,7,11-trimethyldodeca-1,6,10-triene; (6E,10E)-2,6,10-trimethyldodeca-2,6,10-triene; (6E,10Z)-2,6,10-trimethyldodeca-2,6,10-triene

EC Number:

942-471-3

Cas Number:

1581740-29-5

Molecular formula:

C15H26

IUPAC Name:

(6E)-2,6-dimethyl-10-methylidenedodeca-2,6-diene; (6E)-3,7,11-trimethyldodeca-1,6,10-triene; (6E,10E)-2,6,10-trimethyldodeca-2,6,10-triene; (6E,10Z)-2,6,10-trimethyldodeca-2,6,10-triene

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report):RV-873-5-2 "Amyris Renewable Solvent"
- Physical state: Liquid
- Analytical purity: 98.37%
- Lot/batch No.: RV-873-5-2

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: Surface water within 1 mile of water treatment facility

Details on inoculum:

- inoculum: Surface water from Skokie, IL water district.
- proportion and nature of industrial waste water in sewage: unknown, discharge from waste treatment facility within 1
mile.
- test duration and temperature: 28 days or as indicated, 22C +/- 1C
- bacterial inoculum ~1E5 cfu/ml

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 20 mg/L

Based on:

other: Total carbon determined by catalytic oxidation fo the test material

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other: Theoretical oxygen demand

Details on study design:

Study was conducted with 2 replicates.

SAMPLING
- Sampling frequency: continuous; respirometer



CONTROL: Sodium acetate

STATISTICAL METHODS: Sample results are provided as a graph showing the raw data and curvefit analysis.
Determinations of the percent degradation is based on the curve fit analysis performed with guidance from OECD Guidelines for the Testing of
Chemicals / Section 3: Degradation and Accumulation Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in
Wastewater.

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Preliminary study:

na

% Degradation

Parameter:

other: Theoretical oxygen demand

Value:

ca. 99

St. dev.:

10

Sampling time:

28 d

Remarks on result:

other: 10 day window requirement met

Details on results:

Achieved the requirements for Ready Biodegradability by the OECD 301F standard. Achieved a biodegradation plateau by day 28 of the test
period. By curve fit analysis, the achieved plateau was 99% ThO2 +/- SE 10%. the required degradation within the 10-day window was achieved.
OECD 301 ultimate degradation requirement achieved; 60% by day 19

BOD5 / COD results

Results with reference substance:

OECD 301 requirements achieved for sodium acetate; 10 day window (day 3 to day 12)
At 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Test material was readily biodegradable according to results of OECD 301F study.

Executive summary:

Myralene was tested via OECD 301F. Sodium acetate was also tested as a control. The inoculum used was surface water. 60% ThOD biodegradation was achieved by day 19 and within the requisite 10 day window. After 28 days, 99% ThOD was acheived. Test material was readily biodegradable according to results of OECD 301F study.