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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25. Oct 1956 - 19. Nov 1956
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1961
Report date:
1961

Materials and methods

Principles of method if other than guideline:
BASF-Test:
Two animals were treated for 1, 5 or 15 min using occlusive conditions. An application site of 2.5x2.5 cm was covered with the liquid test substance. The animals were observed for 3 weeks and skin changes were recorded daily. For a final evaluation, the findings from 24 and 48 hours from the raw data were taken into account.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-aminopropyldimethylamine
EC Number:
203-680-9
EC Name:
3-aminopropyldimethylamine
Cas Number:
109-55-7
Molecular formula:
C5H14N2
IUPAC Name:
N,N-dimethylpropane-1,3-diamine
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
Vienna White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.6 kg (mean)

Test system

Type of coverage:
occlusive
Preparation of test site:
not specified
Vehicle:
unchanged (no vehicle)
Controls:
other: untreated skin of the same animal served as control
Duration of treatment / exposure:
1, 5 and 15 min
Observation period:
3 weeks
Number of animals:
2
Details on study design:
TEST SITE
- Area of exposure: 2.5x2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): conc. Lutrol and 50 % Lutrol solution
- Time after start of exposure: 1, 5 or 15 min post exposure

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
edema score
Remarks:
15 min exposure
Basis:
mean
Remarks:
animal 1-2
Time point:
24/48 h
Remarks on result:
not determinable
Irritation parameter:
erythema score
Remarks:
15 min exposure
Basis:
mean
Remarks:
animal 1-2
Time point:
24/48 h
Score:
2
Max. score:
4
Reversibility:
fully reversible within: 3 weeks
Remarks on result:
other: After 15 min exposure single haemorrhages occurred. After 8 days bean-sized crusts were seen. At the end of the observation period (3 weeks) increased hair growth was noted.

Any other information on results incl. tables

Mean erythema score after 24 and 48 (animal1/animal 2). 72 h reading is missing.

 Exposure time  same day  24 h  48 h  72 h  8 days  3 weeks
 1 min  1/0 1/0  1/1 -/-  0/0  0/0
 5 min  1/1 2/0 2/2  -/-  0/0 0/0 
 15 min  3/2 2/2 2/2 -/-  0/0  0/0

The original BASF grading was converted into the numerical grading according the OECD Draize system.

After 15 min exposure single haemorrhages occurred. After 8 days bean-sized crusts. At the end of the observation period (3 weeks) increased hair growth was noted.

Applicant's summary and conclusion

Interpretation of results:
Category 1B (corrosive) based on GHS criteria