3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Testing was conducted between 18 June 2009 and 14 July 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Fully GLP and test guideline compliant study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 420 (Acute Oral Toxicity - Fixed Dose Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

EC Number:

700-129-9

Cas Number:

2004-62-8

Molecular formula:

C12H22N4

IUPAC Name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

For the 2,000 mg/kg dose level the test material was used as supplied. For the purpose of the 300 mg/kg dose level sighting test the test material was freshly prepared, as required, as a solution in distilled water.

Doses:

300 and 2,000 mg/kg

No. of animals per sex per dose:

1 female animal for 300 mg/kg dose and 5 female animals for 2,000 mg/kg dose.

Control animals:

no

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

One animal treated at a dose level of 2000 mg/kg was found dead two days after dosing.

Clinical signs:

Signs of systemic toxicity noted in two animals treated at a dose level of 2000 mg/kg were hunched posture, pilo-erection and laboured respiration and/or decreased respiratory rate. These two animals appeared noraml thirteen or fourteen days after dosing. No other signs of systemic toxicity were noted.

Body weight:

Three animals treated at a dose level of 2000 mg/kg showed bodyweight loss during the first week but expected gain in bodyweight during the second week. The remaining surviving animal showed expected gains in bodyweight during the test.

Gross pathology:

Abnormalities noted at necropsy of the animal that died during the study were abnormally red lungs, dark liver and dark kidneys. No abnormalities were noted at necropsy of animals that were killed at the end of the study.

Other findings:

None.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Remarks:

Migrated information

Conclusions:

LD50 > 2000 mg/kg.

Executive summary:

The acute oral median lethal dose (LD50) of the test material in the female Wistar strain rat was estimated to be greater than 2000 mg/kg bodyweight.