Reaction mass of divinylbenzene and ethylstyrene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

The study was not conducted according to guideline/s and GLP but the report contains sufficient data for interpretation of study results

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of divinylbenzene and ethylstyrene

EC Number:

910-757-7

Cas Number:

N/A

Molecular formula:

Divinylbenzene: C10H10 Ethylstyrene: C10H12

IUPAC Name:

Reaction mass of divinylbenzene and ethylstyrene

Details on test material:

Vapor phase chromatograph (VPC) analysis :
Divinylbenzene 55.7%, Ethylvinylbenzene 40.4%, Diethylbenzene 4.5%, Mass 144-146 1.7%, Naphthalene 0.4%, t-Butyl Catechol 1010 ppm, Polymer 4 ppm

The percentage was determined by measuring the percent area of each component shown in the vapor phase chromatograph and is
considered to be approximately equal to weight percent.

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

No data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Twenty-four hours prior to applying the test compound, the entire trunk of twelve rabbits, six males and six females, was clipped free of hair with electric clippers. Doses of 2.0, 3.98 or 7.95 g/kg body weight of the liquid were applied to the skin of the rabbits under a heavy gauge plastic cuff. The cuff was held in place with rubber bands and a cloth bandage taped securely to the marginal hair. At the end of the 24-hour exposure period, the cuffs were removed and the skin was washed with soap and water, rinsed thoroughly and dried with a cloth towel.

Duration of exposure:

24 hours

Doses:

2.0, 3.98 or 7.95 g/kg body weight

No. of animals per sex per dose:

2/sex/dose

Control animals:

no

Details on study design:

The animals were observed for signs of toxicity during the exposure and periodically for two weeks thereafter. Body weights were recorded before and after the 24-hour exposure and at intervals up to 2 weeks post-application or until any initial loss of body weight was regained.

Statistics:

LD50 was calculated in accordance to: Weil CS (1952) . Tables for convenient calculation of median-effective dose (LD50 or ED50) and instructions in their use, Biometries, 8: 249-263.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

8 other: g/kg

95% CL:

4.5 - 11.8

Remarks on result:

other: The LD50 was calculated to be 8.0 g/kg (95% Confidence Limits 4.5 to 11.8 g/kg) with the assumption that a dose of 15.8 g/kg would kill 100% of the animals.

Mortality:

Mortality was observed in two of the four animals at the 7.95 g/kg dosage level. Death of these animals occurred on the third day after dosing. All other animals survived.

Clinical signs:

other: other: other: other: None

Gross pathology:

None

Other findings:

None

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 was calculated to be 8.0 g/kg (95% Confidence Limits 4.5 to 11.8 g/kg).

Executive summary:

An acute dermal study was conducted using methods equivalent to OECD Guideline 402. Twenty-four hours prior to applying the test compound, the entire trunk of twelve rabbits, six males and six females, was clipped free of hair with electric clippers. Doses of 2.0, 3.98 or 7.95 g/kg body weight of the liquid were applied to the skin of the rabbits under a heavy gauge plastic cuff. The cuff was held in place with rubber bands and a cloth bandage taped securely to the marginal hair. At the end of the 24-hour exposure period, the cuffs were removed and the skin was washed with soap and water, rinsed thoroughly and dried with a cloth towel. The animals were observed for signs of toxicity during the exposure and periodically for two weeks thereafter. Body weights were recorded before and after the 24-hour exposure and at intervals up to 2 weeks post-application or until any initial loss of body weight was regained.

 

Mortality was observed in two of the four animals at the 7.95 g/kg dosage level. Death of these animals occurred on the third day after dosing. All other animals survived. Surviving animals lost weight after exposure and returned to starting weight levels slowly. No clinical signs were observed throughout the study. The LD50 was calculated to be 8.0 g/kg (95% Confidence Limits 4.5 to 11.8 g/kg).