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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report which meets basic scientific principles. Study was conducted prior to GLP implementation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
test for acute toxicity after parental application
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
o-anisidine
EC Number:
201-963-1
EC Name:
o-anisidine
Cas Number:
90-04-0
Molecular formula:
C7H9NO
IUPAC Name:
2-methoxyaniline
Details on test material:
- Name of test material (as cited in study report): o-Anisidin

Test animals

Species:
cat
Strain:
not specified
Sex:
female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: sodiume chloride
Doses:
10 and 100 mg/kg bw
No. of animals per sex per dose:
2
Control animals:
no
Details on study design:
- Duration of observation period following administration: 24 h
- Necropsy of survivors performed: yes
- Other examinations performed: haematological investigation

Results and discussion

Applicant's summary and conclusion

Conclusions:
Single intraperitoneal application of 100 mg o-Anisidin per kg bw into cats revealed drastic changes in the blood count (Heinz body formation; methaemoglobin formation; increased leucoyte; reduced lymphocyte count) and is fatal within 7 h. 10 mg/kg bw induced only moderate effects on blood parameters tested and was not fatal. Data support conclusion that lethality is caused by MetHb formation.