Phosphoric acid, iron(2+) lithium salt (1:1:1)

Endpoint summary

Additional information

Short-term toxicity to fish

The substance induced no visible effects in carp at or below an exposure concentration of 28 mg/L, being the concentration measured in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/L (NOEC).

 

The 96h-LC50 based on actual measured concentrations exceeded 28 mg/L, being the expected maximum soluble concentration in the test medium.

 

Long-term toxicity to fish

In accordance with column 2 of REACH annex IX, long-term toxicity tesing on fish testing does not need to be conducted as the chemical safety assessment does not indicate a need to investigate further effects on aquatic organisms. The short term toxicity test with fish showed no effects up to the solubility limit of the test material.

Based on the above, it is considered valid to waive the long-term fish toxicity study.

Short-term toxicity to Daphnia

The substance did not induce acute immobilization of Daphnia magna at 28 mg/L, being the average concentration measured in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/L (NOEC).

 

The 48h-EC50 based on actual measured concentrations exceeded 28 mg/L, being the expected maximum soluble concentration in the test medium.

 

Long-term Toxicity to Daphnia

In accordance with column 2 of REACH annex IX, long-term toxicity tesing on fish testing does not need to be conducted as the chemical safety assessment does not indicate a need to investigate further effects on aquatic organisms. .

In addition, no effects were seen in the short-term Daphnia study at the expected maximum soluble concentration in test medium.

Based on the above, it is considered valid to waive the long-term Daphnia toxicity study.

Toxicity to aquatic algae

Under the conditions of the present study with Selenastrum capricornutum, no significant inhibition of cell growth or reduction of growth rate was recorded at or below an exposure concentration of 24 mg/L, being the concentration measured in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/L (NOEC). Instead, the substance stimulated algal cell growth, which was most likely related to the presence of phosphate in the test substance that served as an extra nutrient source for the algae. In conclusion: the EC50 based on actual measured concentrations for both cell growth inhibition and growth rate reduction exceeded 24 mg/L, being the expected maximum soluble concentration in test medium.

 

Toxicity to microorganisms

The influence of the substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours.

 No significant inhibition of respiration rate of the sludge was recorded at the test material loading rate of 100 mg/L. Therefore, no further testing was needed.

 In conclusion, the substance was not toxic to waste water (activated sludge) bacteria at a loading rate of 100 mg/L. Hence, the EC50 exceeded a loading rate of 100 mg/L.