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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Code of Federal Regulations, Title 16, Section 1500.42
Principles of method if other than guideline:
100 µl phenyl isocyanate was applied into the conjunctival sac of the right eye of 6 rabbits. Reading was carried out 24, 48 and 72 h after start of application of the test substance.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Phenyl isocyanate
EC Number:
203-137-6
EC Name:
Phenyl isocyanate
Cas Number:
103-71-9
Molecular formula:
C7H5NO
IUPAC Name:
isocyanatobenzene

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Duration of treatment / exposure:
8 days (eye was not washed after application of the test substance)
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
6 animals

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24, 48, 72 h
Score:
49.8
Max. score:
110
Reversibility:
not fully reversible within: 8 days
Irritant / corrosive response data:
Eight days after application of the test substance, no repair of the eye damages was observed, with a trend for deterioration. Phenyl isocyanate is therefore highly irritant to the eyes.
Other effects:
No repair of the effects within 8 days, with a trend for further deterioration

Any other information on results incl. tables

Eight days after application of the test substance, no repair of the eye damages was observed, with a trend for deterioration.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Conclusions:
Eight days after application of the test substance, no repair of the eye damages was observed, with a trend for deterioration. Phenyl isocyanate is therefore classified as highly irritant. According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification as Eye Damage 1 is justified.
Executive summary:

100 µl phenylisocyanate was applied into the conjunctival sac of the right eye of 6 rabbits. Reading was carried out 24, 48 and 72 h after start of application of the test substance. A primary irritation score of 49.8 (of 110) was found. Eight days after application of the test substance, no repair of the eye damages was observed, with a trend for deterioration. Phenyl isocyanate is therefore classified as highly irritant.