Ethylenediamine, salt with phosphoric acid

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental report

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance is soluble in ADaMs’ media. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’ media with continuous 15 minutes stirring and the concentration of stock solution was analytically detected and then the test solution was prepared for achieving
the test concentrations of 0.625 mg/L,1.25 mg/L,2.5 mg/L,5 mg/L,10 mg/L ,respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea.
-Strain: straus.
-Source : Own breeding
-Adaptation : Test conditions corresponded to housing conditions
-Food : Selenestrum capricornutum

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

190 (mg of CaCO3)

Test temperature:

20-22 °C

pH:

7.7

Dissolved oxygen:

Above 3 mg/L

Nominal and measured concentrations:

0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml Beaker
- fill volume: 50 mL
- Aeration: no aeration
- Renewal rate of test solution (frequency/flow rate): alternate days
- No. of organisms per vessel: 1 daphnia per vessel
- No. of vessels per concentration (replicates): 10 vessels
- No. of vessels per control (replicates): 10 vessels
-Light intensity: 1000-1500 lux.

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95% CI- 0.927-1.302

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95% CI- 0.175-0.358

Details on results:

First brood observed on 13th day of experiment

Reported statistics and error estimates:

The results obtained was calulated using the Probit analysis

Validity criteria fulfilled:

yes

Conclusions:

The results conclude that test chemical ,median effective concentration and efeective conentration 10 is 1.10 and 0.25 mg/, respectively.

Executive summary:

The study was conducted to evaluate long term toxicity of test material on aquatic invertebrate Daphnia magna, study was performed in accordance with OECD guideline 211 in semi-static condition.The test substance is soluble in ADaMs’ media. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’media with continuous 15 minutes stirring. The test concentrations selected was 0.625 mg/L,1.25 mg/L,2.5 mg/L,5 mg/L,10 mg/L ,respectively.The test was conducted in 100 ml beaker with 50 ml fill volume, one organism was used in each replicates. The daphids were fed with Selenestrum capricornutum algae. First brood observed on 13th day of experiment. Based on nominal concentrations, the EC50 and EC10 was determied to be 1.1 mg/L and 0.25 mg/L, respectively.

Description of key information

Long-term toxicity to aquatic invertebrates:

The study was conducted to evaluate long term toxicity of test material on aquatic invertebrate Daphnia magna, study was performed in accordance with OECD guideline 211 in semi-static condition.The test substance is soluble in ADaMs’ media. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’media with continuous 15 minutes stirring. The test concentrations selected was 0.625 mg/L,1.25 mg/L,2.5 mg/L,5 mg/L,10 mg/L ,respectively.The test was conducted in 100 ml beaker with 50 ml fill volume, one organism was used in each replicates. The daphids were fed with Selenestrum capricornutum algae. First brood observed on 13th day of experiment. Based on nominal concentrations, the EC50 and EC10 was determied to be 1.1 mg/L and 0.25 mg/L, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.25 mg/L

Additional information

Long-term toxicity to aquatic invertebrates:

The study was conducted to evaluate long term toxicity of test material on aquatic invertebrate Daphnia magna, study was performed in accordance with OECD guideline 211 in semi-static condition.The test substance is soluble in ADaMs’ media. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’media with continuous 15 minutes stirring. The test concentrations selected was 0.625 mg/L,1.25 mg/L,2.5 mg/L,5 mg/L,10 mg/L ,respectively.The test was conducted in 100 ml beaker with 50 ml fill volume, one organism was used in each replicates. The daphids were fed with Selenestrum capricornutum algae. First brood observed on 13th day of experiment. Based on nominal concentrations, the EC50 and EC10 was determied to be 1.1 mg/L and 0.25 mg/L, respectively.