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EC number: 203-802-0 | CAS number: 110-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-12-08 to 2009-12-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to a protocol which is comparable to guideline. It is also compliant with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: OECD 439 (2010)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethylthioethanol
- IUPAC Name:
- ethylthioethanol
- Reference substance name:
- 2-(ethylthio)ethanol
- EC Number:
- 203-802-0
- EC Name:
- 2-(ethylthio)ethanol
- Cas Number:
- 110-77-0
- Molecular formula:
- C4H10OS
- IUPAC Name:
- 2-(ethylsulfanyl)ethan-1-ol
Constituent 1
Constituent 2
Test animals
- Species:
- human
- Strain:
- other: not applicable
- Details on test animals or test system and environmental conditions:
- The test uses EPISKIN human epidermis skin constructs consisting of normal, human-derived epidermal keratinocytes, that have been cultured to form a multilayered, highly differentiated model of the human epidermis with a functional multilayered stratum corneum.
Test system
- Type of coverage:
- other: not applicable
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: not applicable
- Amount / concentration applied:
- 10 ul:
TS neat
Negative control = sterile Dulbecco’s Phosphate Buffered Saline (DPBS) with magnesium and calcium.
Positive control was 5% Sodium Dodecyl Sulphate (SDS) in distilled water. - Duration of treatment / exposure:
- 15 minutes
- Observation period:
- not applicable
- Number of animals:
- not applicable
- Details on study design:
The principle of the test is that irritant substances are sufficiently cytotoxic to cause cell death in the cell layers. The cell viability is determined by mitochondrial dehydrogenase activity, assessed by the reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) to a soluble, coloured, formazan product. The prediction model uses the percentage viability values (compared to negative control viability) to identify irritant and non-irritant substances. The test includes acceptance criteria for both negative and positive controls.
After incubation of at least 24 hours in maintenance medium, triplicate tissues were dosed for 15 minutes with test substance, negative or positive control at room temperature. A maximum of four samples were applied in a block with a minimum of 1 minute intervals between each application of substance. On application of 10 μL, the positive control was spread over the tissue for approximately 30 seconds and then respread with a curved flat spatula after 7 minutes application time. After 15 minutes, each tissue was rinsed with 25 mL sterile Dulbeccos Phosphate Buffered Saline (DPBS) to remove residual test substance. Inserts were blotted on absorbent paper to remove remaining DPBS. Each insert was then transferred to a well containing 2 mL maintenance medium and incubated for 42 ± 1 hour at 37 ± 2°C in a humidified atmosphere of 5% CO2 in air.
After 42 ± 1 hour each insert was transferred to a well containing 2 mL of 0.3 mg/mL MTT and incubated for 3 hours ± 5 minutes at 37 ± 2°C in a humidified atmosphere of 5% CO2 in air. At the end of 3 hours ± 5 minutes the triplicate inserts were blotted on absorbent paper. The epidermis was removed from the insert using a biopsy punch, the epidermis separated from the collagen matrix using forceps and both parts placed in a micro tube. When all tissues had been punched, the tissues were vortexed with 500 DL of acidic isopropanol (0.04 N HCl final concentration). The tissues were extracted by storing at 2-8 oC, protected from light, for a minimum of 70 hours. After formazan extraction, duplicate 200 μL aliquots of the extractant from each micro tube were pipetted into the wells of flat-bottomed 96-well plates. The extractant was mixed by vortexing prior to taking the aliquots. The absorbance was read at 540 nm with acidified isopropanol solution as a blank.
The negative control was sterile Dulbecco’s Phosphate Buffered Saline with magnesium and calcium.
The positive control was 5% Sodium Dodecyl Sulphate in distilled water.
Results and discussion
In vivo
- Irritant / corrosive response data:
- MEAN TISSUE VIABILITY (compared to negative control) :
Test substance, ethylthioethanol 77.4% +/-6 (predicted non-irritant)
Positive control 13.3% +/-4 (predicted irritant)
Negative control 100% +/- 9
(Mean tissue viability greater than 50% is considered to indicate non-irritancy.)
Any other information on results incl. tables
No evidence of interaction of test substance with MTT.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- An in vitro study, conducted using EPISKIN (a human epidermis skin construct) in a manner similar to OECD 439 (2010) and with GLP reported mean tissue viability following exposure of 77.4%, indicating that the test material was unlikely to be irritant to the skin.
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