17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Mar - 21 Apr 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

EC Number:

259-700-1

EC Name:

17β-hydroxy-17-(3-hydroxy-1-propynyl)androst-4-ene-3-one

Cas Number:

55542-26-2

Molecular formula:

C22H30O3

IUPAC Name:

(1S,3aS,3bR,9aR,9bS,11aS)-1-hydroxy-1-(3-hydroxyprop-1-yn-1-yl)-9a,11a-dimethyl-1H,2H,3H,3aH,3bH,4H,5H,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-one

Details on test material:

- Name of test material (as cited in study report): Hydroxypropinol
- Analytical purity: 98.9%
- Lot/batch No.: 59002501

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

15 mg/L

Based on:

test mat.

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

0 - 37

Sampling time:

28 d

Details on results:

The test item ZK 4829 was degraded to 0 - 37 % in the triplicates on day 28.

Any other information on results incl. tables



Test item	Nominal
	conc.	1	4	6	8	11	14	18	21	25	28	29
	of carbon
Controll	0 mg/L	3,3	9,4	17,5	23,2	26,2	33,6	42,9	53,2	62,5	72,6	78,1
blank
(mean of 3
vessels)
ZK4829	11,6 mg/L	2	4	4,7	6,5	9,1	11,9	15,1	16,6	16,5	18,1	18,7
(mean of 3
vesse/s)
Reference	10,2 mg/L	8,5	37,7	62,8	74	80,5	90,3	96,3	96,8	97,6	98,5	99,5
(sodium
acetate)
Toxicity control	11,6 mg/L +	12	51,1	69,5	83,2	98,1	107,2	112,5	113,6	115,4	119,3	123,9
(ZK 4829 + sodium acetate)	10,2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

According to the results of the test it can be concluded that ZK 4829'is not readily biodegradable
under the conditions of the test.

Executive summary:

The test item Hydroxypropinol was incubated in an aqueous solution including nutrients with microorganisms

from a municipal sewage treatment plant for 28 days (start of treatment = day 0). The nutrient

solutions were made up of phosphates, magnesium sulphate, iron chloride, ammonium chloride and

calcium chloride.

The test item was incubated at a concentration of 11.6 mg carbon/L (15 mg test item/L) in

triplicate. Additionally, a reference item (sodium acetate) was tested at a concentration of 35 mg/L in

a single set according to the same procedure, in order to verify the viability and activity of the degrading

microorganisms. One further set was incubated with sodium acetate at 10.2 mg carbon/L

(reference item) plus test substance at 11.6 mg carbon/L representing a toxicity control. Furthermore, a

blank control was tested in triplicate without any test or reference item.

The biological degradation of the test and reference items was evaluated by measurement of the

carbon dioxide (C02) produced during the test period. CO2 production was determined on days 1, 4,

6,8, 11, 14, 18, 21, 25, 28 and 29. On day 28 the solutions were acidified in order to expel all dissolved

CO2, and CO2 was determined on day 29. The CO2 production was calculated as the percentage

of total CO2 that the test item could theoretically have produced, based on carbon content.

The test item Hydroxypropinol was degraded to 0 - 37 % in the triplicates on day 28.

The reference item sodium acetate was degraded to 80 % on day 14 and to 89 % on day 28.

In the toxicity control, the reference item (sodium acetate) plus the test item, was degraded

to 52 % on day 28. In accordance with the OECD guideline, the test item is not readily biodegradable under

the conditions of the test and it was not toxic to the microbes of activated sludge.