Registration Dossier
Registration Dossier
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EC number: 294-600-1 | CAS number: 91744-38-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated and gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF.
This indicates that in the range of water solubility the test item was not toxic under the test conditions.
Key value for chemical safety assessment
Additional information
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009.
Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 6.25, 12.5, 25, 50 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
Exposure of Pseudokirchneriella subcapitata to the test item gave EL*50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF. This indicates that in the range of water solubility the test item was not toxic under the test conditions. It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/L. Given that a suitable method of chemical analysis could not be developed to measure the dissolved test item concentration present in the WAFs it was considered appropriate to take samples for Total Organic Carbon (TOC) analysis as an alternative. Analysis of the test preparations at 0 hours showed measured carbon concentration to range from 3.41 to 5.38 mg C/L whilst concentrations in the range of 1.88 to 3.33 mg C/L were obtained at 72 hours.
* EL = Effective Loading Rate
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