Registration Dossier
Registration Dossier
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EC number: 605-751-3 | CAS number: 17514-68-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Key study: Test method according to OECD Guideline 202. GLP study. The EC50 (based on immobilisation) of test item was determined to be 0.030 mg/L in Daphnia magna after 48 hours of exposure.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.03 mg/L
Additional information
Key study: The acute toxicity of the test substance on Daphnia magna was assessed in an Acute immobilisation test over an exposure period of 48 hours in a static, closed system. The study was performed in accordance with the OECD Guideline 202 (GLP study). Based on the results of the performed preliminary range-finding tests, six test concentrations in a geometric series with a separation factor of 2.5 and one untreated control were tested in the main experiment.The nominal concentrations oftest item used in the main experiment were: 0.205, 0.512, 1.28, 3.2, 8 and 20 % saturated solutions. The corresponding calculated and measured geometric mean test item concentrations were: 0.0007, 0.0025, 0.017, 0.046, 1.118, 0.328 mg/L.The difference between the nominal concentrations and the measured concentrations were due to the low solubility of the test item.The biological results are based on the calculated and measured geometric mean concentrations. Twenty animals, divided into four groups (Erlenmeyer flasks with stopper) of five animals each were used at the test and for the control groups. After 48 hours of exposure, the EC50 based on immobilisation was determined to be 0.030 mg/L.
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