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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 02-09, 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-benzyl-1,2-dihydro-3H-indazol-3-one, sodium salt
EC Number:
236-138-5
EC Name:
1-benzyl-1,2-dihydro-3H-indazol-3-one, sodium salt
Cas Number:
13185-09-6
Molecular formula:
C14H12N2O.Na
IUPAC Name:
sodium 1-benzyl-3-oxo-2,3-dihydro-1H-indazol-2-ide
Details on test material:
Name: 1-benzyl-3-hydroxy-1H-indazole sodium salt
Chemical formula : C14H11N2O * Na
Molecular weight : 246.24 g/mol
Batch number : 09-749
Preparation date : February 19, 2010
Expiry date : July 19, 2010
Storage conditions : Room temperature / dark conditions

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The main test included five different concentrations of test item, namely 1.0, 3.2, 10.0, 31.6 and 100.0 mg/L.
The test medium was prepared as follows: prior to the start of the test, a stock solution of 100.0 mg/L was prepared by direct weighing into reconstituted water. The obtained solution showed small white insoluble grains, therefore it was mixed by the mean of a magnetic stirrer for about 4 days to achieve the total solubilisation of test item. The test concentrations were then prepared by taking adequate aliquots of obtained solution and diluting them in aerated reconstituted water.
A negative control with only reconstituted water was tested.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
A strain of the freshwater crustacean Daphnia magna Straus was originally supplied by the Italian Health Department (Istituto Superiore di Sanità) in September 2004. Since that time, the clone has been bred in ChemService laboratories in reconstituted water with the same characteristic to the water used in the tests (as for pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests. The daphnia breeding was daily fed with a fixed amount of green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) cultivated in the ChemService Laboratory and with a suspension of the yeast Saccharomyces cerevisiae.

At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
140-250 mg/L as CaCO3
Test temperature:
The room temperature was in the range 20.3 – 22.3 °C during the test period, these values exceeded the OECD’s recommended range (20 ± 2°C) for a short period, however this deviation did not affect the results of the study as showed by the immobilization data in the control.
pH:
The pH of the test medium after preparation was in the range 7.10 – 7.50 for test item solutions and 7.44 for control, therefore, the pH of the test medium was not adjusted before the start of the test.
Details on test conditions:
The test was performed in 50 mL glass beakers filled with 40 mL of test medium. The beakers were covered with glass plates to reduce the loss of water and to avoid the entry of dust into the solutions.

For each test concentrations and for the control, 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to the test vessels at initiation of the test. The daphnid’s loading rate was greater than one daphnid per 2 mL of test solution.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
IC50
Effect conc.:
41.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 37.1 - 45.6
Results with reference substance (positive control):
At the end of test period, in the negative control 0% of immobilization was observed and any daphnid was found trapped on the test water surface. These values comply with the validity criteria of the test, that provide a maximum immobilization and/or a maximum number of daphnids trapped in to water surface of 10% in the control medium at the end of the test.

Any other information on results incl. tables

Lethal effect of 1-benzyl-3-hydroxy-1-h-indazole sodium salt to Daphnia magna (no.20 daphnia tested):

 Nominal test item concentration (mg/l)  % of immobilized daphnia
 1.0  0 (24 and 48 h)
 3.2  0 (24 and 48 h)
 10.0  0 (24h); 5 (48 h)
 31.6  5 (24 h); 30 (48 h)
 100  40 (24 h); 100 (48 h)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC, LOEC and IC50 values after 24 and 48 h, calculated based on nominal concentrations were are follows:
24 h NOEC: 31.6 mg/l
24h LOEC: 100 mg/l
24 h IC50: > 100.0 mg/l
48 h NOEC: 10 mg/l
48 h LOEC: 31.6 mg/l
48 h IC50: 41.3 (37.1 -45.6) mg/l

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